Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100480 |
| Name | oriT_pOXA-48  |
| Organism | Klebsiella pneumoniae |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_019154 (33088..33193 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pOXA-48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 551 | GenBank | YP_006958827 |
| Name | MobA_pOXA-48 |
UniProt ID | G9I9E5 |
| Length | 658 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 658 a.a. Molecular weight: 75080.76 Da Isoelectric Point: 9.9948
>YP_006958827.1 MobA (plasmid) [Klebsiella pneumoniae]
MIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVDV
EPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLAS
MGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVNDR
QQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKT
FADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAY
TPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSVS
DPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSSR
KKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLTE
SNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQNE
KHRLIREQNNSKNEMQFRSESDDKFNPK
MIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVDV
EPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLAS
MGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVNDR
QQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKT
FADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAY
TPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSVS
DPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSSR
KKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLTE
SNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQNE
KHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G9I9E5 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35989..61868
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HS861_RS00260 (D647_p4063) | 31073..31384 | + | 312 | WP_004187333 | hypothetical protein | - |
| HS861_RS00265 (D647_p4064) | 31517..32053 | + | 537 | WP_011091066 | hypothetical protein | - |
| HS861_RS00270 (D647_p4065) | 32555..32950 | - | 396 | WP_019725163 | hypothetical protein | - |
| HS861_RS00420 | 32949..33512 | + | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| HS861_RS00280 (D647_p4047) | 33499..35478 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HS861_RS00285 (D647_p4046) | 35492..35992 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HS861_RS00290 (D647_p4045) | 35989..36768 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HS861_RS00295 (D647_p4044) | 36779..37942 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HS861_RS00300 | 37932..38192 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HS861_RS00305 | 38217..39569 | + | 1353 | WP_172684945 | toprim domain-containing protein | - |
| HS861_RS00310 | 39532..41508 | + | 1977 | WP_227626110 | LPD7 domain-containing protein | - |
| HS861_RS00315 (D647_p4035) | 41474..41986 | + | 513 | WP_011091071 | hypothetical protein | traL |
| HS861_RS00320 | 41987..42184 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HS861_RS00325 | 42171..42581 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HS861_RS00330 (D647_p4043) | 42580..43362 | + | 783 | WP_015058941 | DotI/IcmL family type IV secretion protein | traM |
| HS861_RS00335 (D647_p4042) | 43371..44522 | + | 1152 | WP_015060001 | DotH/IcmK family type IV secretion protein | traN |
| HS861_RS00340 (D647_p4041) | 44534..45883 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| HS861_RS00345 (D647_p4040) | 45895..46599 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| HS861_RS00350 (D647_p4039) | 46623..47153 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| HS861_RS00355 (D647_p4036) | 47170..47559 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| HS861_RS00360 (D647_p4067) | 47605..48099 | + | 495 | WP_004187480 | hypothetical protein | - |
| HS861_RS00365 (D647_p4038) | 48096..51146 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| HS861_RS00370 (D647_p4037) | 51143..52351 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HS861_RS00375 (D647_p4068) | 52348..52944 | + | 597 | WP_015060003 | hypothetical protein | - |
| HS861_RS00380 (D647_p4069) | 52937..55132 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| HS861_RS00385 (D647_p4070) | 55134..55787 | + | 654 | WP_015060005 | hypothetical protein | - |
| HS861_RS00390 (D647_p4071) | 55856..56098 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
| HS861_RS00395 (D647_p4073) | 56382..57449 | + | 1068 | WP_019725043 | plasmid replication initiator RepA | - |
| HS861_RS00425 | 58672..58767 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| HS861_RS00400 (D647_p4074) | 58818..60905 | - | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| HS861_RS00405 (D647_p4075) | 60918..61868 | - | 951 | WP_004206886 | DsbC family protein | trbB |
Host bacterium
| ID | 941 | GenBank | NC_019154 |
| Plasmid name | pOXA-48 | Incompatibility group | IncL/M |
| Plasmid size | 61881 bp | Coordinate of oriT [Strand] | 33088..33193 [+] |
| Host baterium | Klebsiella pneumoniae |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |