Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   100450
Name   oriT_pEC158 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019076 (5461..5543 [-], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site  
 
 CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pEC158
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   521 GenBank   YP_006953572
Name   MobA_pEC158 insolico UniProt ID   G9BDI5
Length   524 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 524 a.a.        Molecular weight: 58622.16 Da        Isoelectric Point: 6.0836

>YP_006953572.1 MobA (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLERADRTLEPVIQRELEIR
EERLIQEREHVLSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

(57-237)


  Protein structure


Source ID Structure
AlphaFold DB G9BDI5


Host bacterium


ID   911 GenBank   NC_019076
Plasmid name   pEC158 Incompatibility group   ColRNAI
Plasmid size   5706 bp Coordinate of oriT [Strand]   5461..5543 [-]
Host baterium   Escherichia coli

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -