Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100449
Name   oriT_pHN7A8 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019073 (40087..40556 [-], 470 nt)
oriT length   470 nt
IRs (inverted repeats)      142..151, 154..163  (ATAGGGTCGT..ACGCCCCTAT)
 231..238, 241..248  (GCAAAAAC..GTTTTTGC)
Location of nic site      257..258
Conserved sequence flanking the
  nic site    		  GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 470 nt

>oriT_pHN7A8
AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCCTAATATATCCAAATGTTTTTCATAAAATTTGTCAGTACTGACCCTAACAAGGGTCGCCATAGGGTCGCCATAGGGTCGTCAACGCCCCTATTTAATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGCTAATGATTCAAAAAGCAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   520 GenBank   YP_006953553
Name   TraI_pHN7A8 insolico UniProt ID   G9G1D7
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192132.90 Da        Isoelectric Point: 6.0788

>YP_006953553.1 type IV secretion-like conjugative transfer relaxase protein TraI (plasmid) [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAENRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLPEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREIDA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQVRERAAITERETALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 39699..67887

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS818_RS00235 (pHN7A8_049) 35671..36105 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
HS818_RS00240 (pHN7A8_050) 36102..36864 + 763 Protein_48 plasmid SOS inhibition protein A -
HS818_RS00495 (pHN7A8_051) 37043..37192 + 150 Protein_49 plasmid maintenance protein Mok -
HS818_RS00245 (pHN7A8_052) 37134..37259 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
HS818_RS00250 (pHN7A8_053) 37578..37874 - 297 Protein_51 hypothetical protein -
HS818_RS00255 (pHN7A8_054) 38174..38470 + 297 WP_001272251 hypothetical protein -
HS818_RS00260 (pHN7A8_055) 38581..39402 + 822 WP_001234445 DUF932 domain-containing protein -
HS818_RS00265 (pHN7A8_057) 39699..40346 - 648 WP_015059008 transglycosylase SLT domain-containing protein virB1
HS818_RS00270 (pHN7A8_058) 40623..41006 + 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
HS818_RS00275 (pHN7A8_059) 41197..41883 + 687 WP_015059009 PAS domain-containing protein -
HS818_RS00280 (pHN7A8_060) 41977..42204 + 228 WP_001254386 conjugal transfer relaxosome protein TraY -
HS818_RS00285 (pHN7A8_061) 42238..42603 + 366 WP_015059010 type IV conjugative transfer system pilin TraA -
HS818_RS00290 (pHN7A8_062) 42618..42929 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
HS818_RS00295 (pHN7A8_063) 42951..43517 + 567 WP_000399794 type IV conjugative transfer system protein TraE traE
HS818_RS00300 (pHN7A8_064) 43504..44232 + 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
HS818_RS00305 (pHN7A8_065) 44232..45659 + 1428 WP_015059011 F-type conjugal transfer pilus assembly protein TraB traB
HS818_RS00310 (pHN7A8_066) 45649..46239 + 591 WP_000002778 conjugal transfer pilus-stabilizing protein TraP -
HS818_RS00315 (pHN7A8_067) 46232..46423 + 192 WP_032072783 conjugal transfer protein TrbD -
HS818_RS00320 (pHN7A8_068) 46435..46686 + 252 WP_001038341 conjugal transfer protein TrbG -
HS818_RS00325 (pHN7A8_069) 46683..47198 + 516 WP_000809902 type IV conjugative transfer system lipoprotein TraV traV
HS818_RS00330 (pHN7A8_070) 47333..47554 + 222 WP_001278692 conjugal transfer protein TraR -
HS818_RS00335 (pHN7A8_072) 47714..50341 + 2628 WP_015059012 type IV secretion system protein TraC virb4
HS818_RS00340 (pHN7A8_073) 50338..50724 + 387 WP_015059013 type-F conjugative transfer system protein TrbI -
HS818_RS00345 (pHN7A8_074) 50721..51353 + 633 WP_001203735 type-F conjugative transfer system protein TraW traW
HS818_RS00350 (pHN7A8_075) 51350..52342 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
HS818_RS00355 (pHN7A8_076) 52369..52677 + 309 WP_000412447 hypothetical protein -
HS818_RS00360 (pHN7A8_077) 52740..53261 + 522 WP_015059014 hypothetical protein -
HS818_RS00365 (pHN7A8_078) 53288..53926 + 639 WP_000087532 type-F conjugative transfer system pilin assembly protein TrbC trbC
HS818_RS00370 (pHN7A8_079) 53923..54294 + 372 WP_000056336 hypothetical protein -
HS818_RS00375 (pHN7A8_080) 54320..54742 + 423 WP_001229309 HNH endonuclease signature motif containing protein -
HS818_RS00380 (pHN7A8_081) 54739..56589 + 1851 WP_015059015 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HS818_RS00385 (pHN7A8_082) 56616..56873 + 258 WP_053913405 conjugal transfer protein TrbE -
HS818_RS00390 (pHN7A8_083) 56866..57609 + 744 WP_015059017 type-F conjugative transfer system pilin assembly protein TraF traF
HS818_RS00395 (pHN7A8_084) 57770..58006 + 237 WP_000270010 type-F conjugative transfer system pilin chaperone TraQ -
HS818_RS00400 (pHN7A8_085) 57993..58538 + 546 WP_000059817 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HS818_RS00405 (pHN7A8_086) 58528..58842 + 315 WP_001384255 P-type conjugative transfer protein TrbJ -
HS818_RS00410 (pHN7A8_087) 58796..59188 + 393 WP_000126338 F-type conjugal transfer protein TrbF -
HS818_RS00415 (pHN7A8_088) 59175..60548 + 1374 WP_000944325 conjugal transfer pilus assembly protein TraH traH
HS818_RS00420 (pHN7A8_089) 60545..63361 + 2817 WP_015059018 conjugal transfer mating-pair stabilization protein TraG traG
HS818_RS00425 (pHN7A8_090) 63394..63915 + 522 WP_000632668 conjugal transfer entry exclusion protein TraS -
HS818_RS00430 (pHN7A8_091) 63940..64671 + 732 WP_021559024 conjugal transfer complement resistance protein TraT -
HS818_RS00435 (pHN7A8_092) 64874..65611 + 738 WP_015059020 hypothetical protein -
HS818_RS00440 (pHN7A8_093) 65662..67887 + 2226 WP_000009363 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   910 GenBank   NC_019073
Plasmid name   pHN7A8 Incompatibility group   IncFII
Plasmid size   76878 bp Coordinate of oriT [Strand]   40087..40556 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   blaCTX-M-65, fosA3, blaTEM-1B, rmtB
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -