Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100444
Name   oriT_pPG010208 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019065 (56095..56645 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pPG010208
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   515 GenBank   YP_006952766
Name   TraI_pPG010208 insolico UniProt ID   G0XAC3
Length   992 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>YP_006952766.1 putative type IV conjugative transfer system protein TraI (plasmid) [Escherichia coli]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB G0XAC3


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54229..79420

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS730_RS00380 50254..50487 - 234 WP_001191892 hypothetical protein -
HS730_RS00385 (pPG010208_67) 50469..51086 - 618 WP_001249396 hypothetical protein -
HS730_RS00390 (pPG010208_68) 51254..54232 + 2979 WP_001337757 MobH family relaxase -
HS730_RS00395 (pPG010208_69) 54229..56094 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
HS730_RS00400 (pPG010208_70) 56105..56689 + 585 WP_000332866 hypothetical protein -
HS730_RS00405 (pPG010208_71) 56646..57275 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
HS730_RS00410 (pPG010208_72) 57285..57731 + 447 WP_000122506 hypothetical protein -
HS730_RS00415 (pPG010208_73) 57741..58118 + 378 WP_000869261 hypothetical protein -
HS730_RS00420 (pPG010208_74) 58118..58780 + 663 WP_001231463 hypothetical protein -
HS730_RS00425 58961..59104 + 144 WP_001275801 hypothetical protein -
HS730_RS00430 (pPG010208_75) 59116..59481 + 366 WP_001052531 hypothetical protein -
HS730_RS00435 (pPG010208_76) 59626..59907 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HS730_RS00440 (pPG010208_77) 59904..60530 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
HS730_RS00445 (pPG010208_78) 60514..61431 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HS730_RS00450 (pPG010208_79) 61431..62744 + 1314 WP_024131605 TraB/VirB10 family protein traB
HS730_RS00455 (pPG010208_80) 62741..63319 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HS730_RS00460 (pPG010208_81) 63323..63715 + 393 WP_000479535 TraA family conjugative transfer protein -
HS730_RS00465 (pPG010208_82) 63916..69447 + 5532 WP_000606833 Ig-like domain-containing protein -
HS730_RS00470 (pPG010208_83) 69596..70303 + 708 WP_001259347 DsbC family protein -
HS730_RS00475 (pPG010208_84) 70300..72747 + 2448 WP_000637386 type IV secretion system protein TraC virb4
HS730_RS00480 (pPG010208_85) 72762..73079 + 318 WP_000351984 hypothetical protein -
HS730_RS00485 (pPG010208_86) 73076..73606 + 531 WP_001010738 S26 family signal peptidase -
HS730_RS00490 (pPG010208_87) 73569..74834 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
HS730_RS00495 (pPG010208_88) 74831..75502 + 672 WP_015058966 EAL domain-containing protein -
HS730_RS00500 (pPG010208_89) 75502..76506 + 1005 WP_043940352 TraU family protein traU
HS730_RS00505 (pPG010208_90) 76622..79420 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
HS730_RS00510 (pPG010208_91) 79459..80319 - 861 WP_000709517 hypothetical protein -
HS730_RS00515 (pPG010208_92) 80442..81083 - 642 WP_000796664 hypothetical protein -
HS730_RS00520 (pPG010208_93) 81377..81697 + 321 WP_000547566 hypothetical protein -
HS730_RS00525 82001..82186 + 186 WP_001186917 hypothetical protein -
HS730_RS00530 (pPG010208_94) 82406..83374 + 969 WP_000085162 AAA family ATPase -
HS730_RS00535 (pPG010208_95) 83385..84293 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   905 GenBank   NC_019065
Plasmid name   pPG010208 Incompatibility group   IncA/C2
Plasmid size   135803 bp Coordinate of oriT [Strand]   56095..56645 [+]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   mph(E), msr(E), floR, tet(A), aph(6)-Id, aph(3'')-Ib, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -