Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100433
• Name: oriT_pHUSEC41-3
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_018997 (827..914 [-], 88 nt)
• oriT length: 88 nt
• IRs (inverted repeats): 8..17, 21..30 (GTTTCGGGGC..GCCCTGAACC)
• Location of nic site: 61..62
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 88 nt

>oriT_pHUSEC41-3
TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGTGCAGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_pHUSEC41-3

• ID: 504
• GenBank: YP_006940173
• Name: Mob_pHUSEC41-3
• UniProt ID: G4VUT8
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57853.18 Da
• Isoelectric Point: 6.0398

>YP_006940173.1 plasmid mobilization protein (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGDPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRR
YSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGYPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHELSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: G4VUT8

Host bacterium

• ID: 894
• GenBank: NC_018997
• Plasmid name: pHUSEC41-3
• Incompatibility group: ColRNAI
• Plasmid size: 7930 bp
• Coordinate of oriT [Strand]: 827..914 [-]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -