Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100431 |
Name | oriT_pEAR4.3 |
Organism | Erwinia amylovora ATCC BAA-2158 |
Sequence Completeness | intact |
NCBI accession of oriT (coordinates [strand]) | NC_018977 (3850..4034 [+], 185 nt) |
oriT length | 185 nt |
IRs (inverted repeats) | 14..21, 25..32 (TTCGGGGC..GCCCTGAA) |
Location of nic site | 65..66 |
Conserved sequence flanking the nic site |
CTGG|CTTA |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 185 nt
>oriT_pEAR4.3
GGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAATATGTTACGGCGAAGTGGTGATTAATGGAAGTGCTTCATGTGGCAGGGGAAATATGTGACTCAGGAGTGACCGCAATACGATGTACGGACAGATGAAATGACGCCTCCTCGCTCA
GGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAATATGTTACGGCGAAGTGGTGATTAATGGAAGTGCTTCATGTGGCAGGGGAAATATGTGACTCAGGAGTGACCGCAATACGATGTACGGACAGATGAAATGACGCCTCCTCGCTCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 502 | GenBank | YP_006939437 |
Name | MbeA_pEAR4.3 | UniProt ID | E5BAY8 |
Length | 509 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 509 a.a. Molecular weight: 56080.31 Da Isoelectric Point: 7.0160
>YP_006939437.1 Mobilization protein mbeA (plasmid) [Erwinia amylovora ATCC BAA-2158]
MQCWRRGGMMIVKFHARGRGGGSGPVDYLLGKERNREGACVLQGRPEEVRELIDASPYAKKYTSGVLSFA
EADLPFGQREKLMESFERVLMPGLDKDQYSILWVEHRDKGRLELNFVIPNTELLTGKRLQPYFDRADRPR
IDAWQTIVNDLLELHDPNAPENRRALVTPGSLPESKQEAARAITRGLLALASSGELKTRQDVTDALTASG
FDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRVQRARAVCKSGTE
RKREENQRRHQRPRAGYDLGHAVEQAERSAHGGPDVADHHDGVSAAYRQPERDTVVPGAAHRLEPGDHPG
TGADAGHAVGEEQRGTAFDVRGQQTALREGEPGSGEVRRGFGLDDTGGEIEHDGAGKTVAERVRAATAGL
LAKAGRVGERLRGMAEDVWSYATGERGAERSRIGLEQAGAEFERAASPLVDRLNVIEQRREHERALQHQK
TLELERSQRQRTYDGPSLG
MQCWRRGGMMIVKFHARGRGGGSGPVDYLLGKERNREGACVLQGRPEEVRELIDASPYAKKYTSGVLSFA
EADLPFGQREKLMESFERVLMPGLDKDQYSILWVEHRDKGRLELNFVIPNTELLTGKRLQPYFDRADRPR
IDAWQTIVNDLLELHDPNAPENRRALVTPGSLPESKQEAARAITRGLLALASSGELKTRQDVTDALTASG
FDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRVQRARAVCKSGTE
RKREENQRRHQRPRAGYDLGHAVEQAERSAHGGPDVADHHDGVSAAYRQPERDTVVPGAAHRLEPGDHPG
TGADAGHAVGEEQRGTAFDVRGQQTALREGEPGSGEVRRGFGLDDTGGEIEHDGAGKTVAERVRAATAGL
LAKAGRVGERLRGMAEDVWSYATGERGAERSRIGLEQAGAEFERAASPLVDRLNVIEQRREHERALQHQK
TLELERSQRQRTYDGPSLG
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | E5BAY8 |
Host bacterium
ID | 892 | GenBank | NC_018977 |
Plasmid name | pEAR4.3 | Incompatibility group | ColRNAI |
Plasmid size | 4369 bp | Coordinate of oriT [Strand] | 3850..4034 [+] |
Host baterium | Erwinia amylovora ATCC BAA-2158 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |