Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100431
Name   oriT_pEAR4.3 in_silico
Organism   Erwinia amylovora ATCC BAA-2158
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_018977 (3850..4034 [+], 185 nt)
oriT length   185 nt
IRs (inverted repeats)      14..21, 25..32  (TTCGGGGC..GCCCTGAA)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 185 nt

>oriT_pEAR4.3
GGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAATATGTTACGGCGAAGTGGTGATTAATGGAAGTGCTTCATGTGGCAGGGGAAATATGTGACTCAGGAGTGACCGCAATACGATGTACGGACAGATGAAATGACGCCTCCTCGCTCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   502 GenBank   YP_006939437
Name   MbeA_pEAR4.3 insolico UniProt ID   E5BAY8
Length   509 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 509 a.a.        Molecular weight: 56080.31 Da        Isoelectric Point: 7.0160

>YP_006939437.1 Mobilization protein mbeA (plasmid) [Erwinia amylovora ATCC BAA-2158]
MQCWRRGGMMIVKFHARGRGGGSGPVDYLLGKERNREGACVLQGRPEEVRELIDASPYAKKYTSGVLSFA
EADLPFGQREKLMESFERVLMPGLDKDQYSILWVEHRDKGRLELNFVIPNTELLTGKRLQPYFDRADRPR
IDAWQTIVNDLLELHDPNAPENRRALVTPGSLPESKQEAARAITRGLLALASSGELKTRQDVTDALTASG
FDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRVQRARAVCKSGTE
RKREENQRRHQRPRAGYDLGHAVEQAERSAHGGPDVADHHDGVSAAYRQPERDTVVPGAAHRLEPGDHPG
TGADAGHAVGEEQRGTAFDVRGQQTALREGEPGSGEVRRGFGLDDTGGEIEHDGAGKTVAERVRAATAGL
LAKAGRVGERLRGMAEDVWSYATGERGAERSRIGLEQAGAEFERAASPLVDRLNVIEQRREHERALQHQK
TLELERSQRQRTYDGPSLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(67-247)


  Protein structure


Source ID Structure
AlphaFold DB E5BAY8


Host bacterium

ID   892 GenBank   NC_018977
Plasmid name   pEAR4.3 Incompatibility group   ColRNAI
Plasmid size   4369 bp Coordinate of oriT [Strand]   3850..4034 [+]
Host baterium   Erwinia amylovora ATCC BAA-2158

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -