Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100428
• Name: oriT_pAA-EA11
• Organism: Escherichia coli O104:H4 str. 2011C-3493
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_018666 (52043..52492 [+], 450 nt)
• oriT length: 450 nt
• IRs (inverted repeats): IR1: 201..215, 222..236 (ATTCATTGGTGAATC..GATTCACCAATGAAT) IR2: 308..315, 318..325 (GCAAAAAC..GTTTTTGC)
• Location of nic site: 334..335
• Conserved sequence flanking the nic site: GTGGGGTGT|GG
• Note: predicted by the oriTfinder

oriT sequence

• Length: 450 nt

>oriT_pAA-EA11
ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGACAATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAATCACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGATTCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTTTTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAGCAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

TraI_pAA-EA11

• ID: 499
• GenBank: YP_006792604
• Name: TraI_pAA-EA11
• UniProt ID: A0A0E0Y6S8
• Length: 1237 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 1237 a.a.
• Molecular weight: 134489.20 Da
• Isoelectric Point: 5.9884

>YP_006792604.1 conjugal transfer nickase/helicase TraI (plasmid) [Escherichia coli O104:H4 str. 2011C-3493]
MAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQDGMTFTPGSTVIVDQGEKLSLKET
LTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFA
ASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQ
WNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKTPGLR
VSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKAGLHTPAQQAI
HLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYE
AEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILEASDRFTVVQGYAGVGKTTQFRAVMSA
VNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNT
DMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDV
ERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTG
RTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALA
LVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVA
NSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAG
FESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDVAAGR
AVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGEGRVKGSED
AQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAG
NGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE

Protein domains

Predicted by InterproScan.

• single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N) (127-178)

• DNA helicase TraI (TraI_C) (986-1109)

• DNA relaxase TraI 2B/2B-like domain (TraI_2B) (185-264)

• AAA domain (AAA_30) (520-708)

Protein structure

• AlphaFold DB: A0A0E0Y6S8

Host bacterium

• ID: 889
• GenBank: NC_018666
• Plasmid name: pAA-EA11
• Incompatibility group: IncFIB
• Plasmid size: 74217 bp
• Coordinate of oriT [Strand]: 52043..52492 [+]
• Host baterium: Escherichia coli O104:H4 str. 2011C-3493

Cargo genes

• Drug resistance gene: -
• Virulence gene: aap/aspU, aggA, aggB, aggC, aggD
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -