Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   100427
Name   oriT_pAA-09EL71 in_silico
Organism   Escherichia coli O104:H4 str. 2009EL-2071
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_018662 (24288..24737 [+], 450 nt)
oriT length   450 nt
IRs (inverted repeats)      IR1: 201..215, 222..236  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
 IR2: 308..315, 318..325  (GCAAAAAC..GTTTTTGC)
Location of nic site      334..335
Conserved sequence flanking the
  nic site  
 
 GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 450 nt

>oriT_pAA-09EL71
ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGACAATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAATCACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGATTCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTTTTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAGCAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   498 GenBank   WP_001390493
Name   TraI_pAA-09EL71 insolico UniProt ID   _
Length   1231 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1231 a.a.        Molecular weight: 133814.33 Da        Isoelectric Point: 6.0682

>WP_001390493.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQDGMTFTPGSTVIVDQGEKLSLKETLTLLDG
AARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAG
EESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETR
SHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKTPGLRVSGGDR
LQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNG
LARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKAGLHTPAQQAIHLALPV
LESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSIL
RHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILEASDRFTVVQGYAGVGKTTQFRAVMSAVNMLPA
SERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAY
ALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSG
LESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAR
EQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALALVDSVY
HRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTV
TAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYV
ALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDVAAGRAVLRQA
GLSGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVAL
QGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVT
AEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE

  Protein domains


Predicted by InterproScan.

(121-172)

(514-702)

(980-1103)

(179-258)


  Protein structure



No available structure.




Host bacterium


ID   888 GenBank   NC_018662
Plasmid name   pAA-09EL71 Incompatibility group   IncFIB
Plasmid size   75573 bp Coordinate of oriT [Strand]   24288..24737 [+]
Host baterium   Escherichia coli O104:H4 str. 2009EL-2071

Cargo genes


Drug resistance gene   -
Virulence gene   aap/aspU, aggA, aggB, aggC, aggD
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -