Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100426 |
| Name | oriT_pESBL-EA11  |
| Organism | Escherichia coli O104:H4 str. 2011C-3493 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_018659 (70345..70454 [-], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_pESBL-EA11
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 497 | GenBank | YP_006781790 |
| Name | Relaxase_pESBL-EA11 |
UniProt ID | A0A0E0Y828 |
| Length | 899 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 103861.32 Da Isoelectric Point: 7.4288
>YP_006781790.1 relaxase (plasmid) [Escherichia coli O104:H4 str. 2011C-3493]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNRGGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNRGGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y828 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1567..26761
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| O3K_RS25620 (O3K_25577) | 1567..2367 | - | 801 | WP_001164783 | IncI1-type conjugal transfer protein TraT | traT |
| O3K_RS25625 (O3K_25582) | 2351..2539 | - | 189 | WP_001277253 | putative conjugal transfer protein TraS | - |
| O3K_RS25630 (O3K_25587) | 2603..3007 | - | 405 | WP_000086965 | IncI1-type conjugal transfer protein TraR | traR |
| O3K_RS25635 (O3K_25592) | 3058..3585 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| O3K_RS25640 (O3K_25597) | 3585..4289 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| O3K_RS25645 (O3K_25602) | 4289..5578 | - | 1290 | WP_001271994 | conjugal transfer protein TraO | traO |
| O3K_RS25650 (O3K_25607) | 5581..6564 | - | 984 | WP_001191879 | IncI1-type conjugal transfer protein TraN | traN |
| O3K_RS25655 (O3K_25612) | 6575..7267 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| O3K_RS25660 (O3K_25617) | 7264..7611 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| O3K_RS25665 (O3K_25622) | 7629..11393 | - | 3765 | WP_001141540 | LPD7 domain-containing protein | - |
| O3K_RS25670 (O3K_25627) | 11483..12034 | - | 552 | WP_000014586 | phospholipase D family protein | - |
| O3K_RS25675 | 12049..12339 | - | 291 | WP_001314267 | hypothetical protein | traK |
| O3K_RS25680 (O3K_25632) | 12336..13484 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| O3K_RS25685 (O3K_25637) | 13481..14299 | - | 819 | WP_000646098 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| O3K_RS25690 (O3K_25642) | 14296..14754 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| O3K_RS25695 (O3K_25647) | 15149..15733 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| O3K_RS25700 (O3K_25652) | 15793..16995 | - | 1203 | WP_000976357 | conjugal transfer protein TraF | - |
| O3K_RS25705 (O3K_25657) | 17081..17905 | - | 825 | WP_001238932 | conjugal transfer protein TraE | traE |
| O3K_RS25710 (O3K_25662) | 18056..19210 | - | 1155 | WP_001139956 | site-specific integrase | - |
| O3K_RS27260 | 19209..19562 | + | 354 | WP_001393368 | hypothetical protein | - |
| O3K_RS27505 | 20623..20847 | + | 225 | Protein_21 | hypothetical protein | - |
| O3K_RS25725 (O3K_25677) | 20844..22280 | - | 1437 | WP_000527428 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| O3K_RS25730 (O3K_25682) | 22268..22924 | - | 657 | WP_001193549 | A24 family peptidase | - |
| O3K_RS25735 (O3K_25687) | 22909..23469 | - | 561 | WP_000014005 | lytic transglycosylase domain-containing protein | virB1 |
| O3K_RS25740 (O3K_25692) | 23479..24093 | - | 615 | WP_000908227 | type 4 pilus major pilin | - |
| O3K_RS25745 (O3K_25697) | 24110..25195 | - | 1086 | WP_001208802 | type II secretion system F family protein | - |
| O3K_RS25750 (O3K_25702) | 25208..26761 | - | 1554 | WP_000362206 | ATPase, T2SS/T4P/T4SS family | virB11 |
| O3K_RS25755 (O3K_25707) | 26772..27224 | - | 453 | WP_001247337 | type IV pilus biogenesis protein PilP | - |
| O3K_RS25760 (O3K_25712) | 27211..28506 | - | 1296 | WP_000752782 | type 4b pilus protein PilO2 | - |
| O3K_RS25765 (O3K_25717) | 28499..30181 | - | 1683 | WP_000748144 | PilN family type IVB pilus formation outer membrane protein | - |
| O3K_RS25770 (O3K_25722) | 30195..30632 | - | 438 | WP_000539806 | type IV pilus biogenesis protein PilM | - |
| O3K_RS25775 (O3K_25727) | 30632..31699 | - | 1068 | WP_001360287 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 887 | GenBank | NC_018659 |
| Plasmid name | pESBL-EA11 | Incompatibility group | IncI1 |
| Plasmid size | 88544 bp | Coordinate of oriT [Strand] | 70345..70454 [-] |
| Host baterium | Escherichia coli O104:H4 str. 2011C-3493 |
Cargo genes
| Drug resistance gene | blaCTX-M-15, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |