Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100421
• Name: oriT_p52
• Organism: Escherichia coli ETEC H10407
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_017721 (4842..4926 [+], 85 nt)
• oriT length: 85 nt
• IRs (inverted repeats): _
• Location of nic site: 58..59
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 85 nt

>oriT_p52
GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCAGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_p52

• ID: 492
• GenBank: WP_000635817
• Name: Nuclease_p52
• UniProt ID: E3PP05
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57554.03 Da
• Isoelectric Point: 6.8427

>WP_000635817.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKVLPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRR
YSPVHNVDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHALSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (59-237)

Protein structure

• AlphaFold DB: E3PP05

Host bacterium

• ID: 882
• GenBank: NC_017721
• Plasmid name: p52
• Incompatibility group: _
• Plasmid size: 5175 bp
• Coordinate of oriT [Strand]: 4842..4926 [+]
• Host baterium: Escherichia coli ETEC H10407

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -