Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100413 |
| Name | oriT_pO83_CORR  |
| Organism | Escherichia coli O83:H1 str. NRG 857C |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_017659 (100717..101006 [+], 290 nt) |
| oriT length | 290 nt |
| IRs (inverted repeats) | 225..232, 235..242 n (GCAAAAAC..GTTTTTGC) |
| Location of nic site | 251..252 |
| Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 290 nt
>oriT_pO83_CORR
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 484 | GenBank | YP_006162275 |
| Name | TraI_pO83_CORR |
UniProt ID | A0A0H3EQ99 |
| Length | 1756 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192164.99 Da Isoelectric Point: 5.8953
>YP_006162275.1 conjugative transfer relaxase protein TraI (plasmid) [Escherichia coli O83:H1 str. NRG 857C]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KVRAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEETSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGIGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESTYVALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGCVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTGQAVREIAWQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVRDLQKE
KTLGGD
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KVRAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEETSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGIGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESTYVALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGCVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTGQAVREIAWQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 89054..101568
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NRG857_RS23230 (NRG857_30178) | 88420..88647 | + | 228 | WP_000450520 | toxin-antitoxin system antitoxin VapB | - |
| NRG857_RS23235 (NRG857_30179) | 88647..89045 | + | 399 | WP_000911313 | type II toxin-antitoxin system VapC family toxin | - |
| NRG857_RS23240 (NRG857_30181) | 89054..91207 | - | 2154 | WP_000009324 | type IV conjugative transfer system coupling protein TraD | virb4 |
| NRG857_RS23245 (NRG857_30184) | 91460..92191 | - | 732 | WP_023565183 | conjugal transfer complement resistance protein TraT | - |
| NRG857_RS23250 (NRG857_30186) | 92223..92720 | - | 498 | WP_000605857 | entry exclusion protein | - |
| NRG857_RS23255 (NRG857_30187) | 92736..95558 | - | 2823 | WP_001007018 | conjugal transfer mating-pair stabilization protein TraG | traG |
| NRG857_RS24085 | 95555..95935 | - | 381 | Protein_98 | conjugal transfer protein TraH | - |
| NRG857_RS24090 | 96017..96865 | - | 849 | Protein_99 | F-type conjugal transfer pilus assembly protein TraB | - |
| NRG857_RS23265 (NRG857_30189) | 96865..97593 | - | 729 | WP_001230787 | type-F conjugative transfer system secretin TraK | traK |
| NRG857_RS23270 (NRG857_30191) | 97580..98146 | - | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
| NRG857_RS23275 (NRG857_30193) | 98168..98479 | - | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
| NRG857_RS23280 (NRG857_30194) | 98494..98859 | - | 366 | WP_000994779 | type IV conjugative transfer system pilin TraA | - |
| NRG857_RS23285 (NRG857_30195) | 98892..99287 | - | 396 | WP_014640559 | conjugal transfer relaxosome DNA-bindin protein TraY | - |
| NRG857_RS23290 (NRG857_30197) | 99386..100075 | - | 690 | WP_000283385 | conjugal transfer transcriptional regulator TraJ | - |
| NRG857_RS23295 (NRG857_30199) | 100262..100645 | - | 384 | WP_001151524 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| NRG857_RS23300 (NRG857_30200) | 100978..101568 | + | 591 | WP_001376243 | transglycosylase SLT domain-containing protein | virB1 |
| NRG857_RS23305 (NRG857_30203) | 101864..102685 | - | 822 | WP_001234426 | DUF932 domain-containing protein | - |
| NRG857_RS23310 (NRG857_30204) | 102803..103090 | - | 288 | WP_000107535 | hypothetical protein | - |
| NRG857_RS23950 (NRG857_30206) | 103115..103321 | - | 207 | WP_000547968 | hypothetical protein | - |
| NRG857_RS23955 | 103391..103563 | + | 173 | Protein_111 | hypothetical protein | - |
| NRG857_RS23960 | 103561..103791 | - | 231 | WP_071586998 | hypothetical protein | - |
| NRG857_RS23320 (NRG857_30210) | 104011..104136 | - | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
| NRG857_RS23965 (NRG857_30210) | 104078..104227 | - | 150 | Protein_114 | plasmid maintenance protein Mok | - |
| NRG857_RS23325 (NRG857_30212) | 104249..104437 | + | 189 | WP_001299721 | hypothetical protein | - |
| NRG857_RS23330 (NRG857_30216) | 104406..105168 | - | 763 | Protein_116 | plasmid SOS inhibition protein A | - |
| NRG857_RS23335 (NRG857_30217) | 105165..105599 | - | 435 | WP_000845936 | conjugation system SOS inhibitor PsiB | - |
| NRG857_RS23340 | 105654..105851 | - | 198 | Protein_118 | hypothetical protein | - |
| NRG857_RS23345 (NRG857_30220) | 105879..106112 | - | 234 | WP_000005990 | DUF905 family protein | - |
Host bacterium
| ID | 874 | GenBank | NC_017659 |
| Plasmid name | pO83_CORR | Incompatibility group | IncFIC |
| Plasmid size | 147060 bp | Coordinate of oriT [Strand] | 100717..101006 [+] |
| Host baterium | Escherichia coli O83:H1 str. NRG 857C |
Cargo genes
| Drug resistance gene | tet(A), catA1, blaTEM-1B, sul2, aph(3'')-Ib, aph(6)-Id, dfrA1, ant(3'')-Ia, qacE, sul1, mph(B), sitABCD |
| Virulence gene | iroB, iroC, iroD, iroE, iroN, iutA, iucC, iucB, iucA |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |