Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100411
• Name: oriT_p12579_3
• Organism: Escherichia coli O55:H7 str. RM12579
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_017654 (8552..8634 [-], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_p12579_3
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_p12579_3

• ID: 482
• GenBank: WP_000635795
• Name: Nuclease_p12579_3
• UniProt ID: _
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57821.27 Da
• Isoelectric Point: 6.3779

>WP_000635795.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRR
YSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGPFPVYREWRDALVSWRADTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHELSLERERQLEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

No available structure.

Host bacterium

• ID: 872
• GenBank: NC_017654
• Plasmid name: p12579_3
• Incompatibility group: ColRNAI
• Plasmid size: 12068 bp
• Coordinate of oriT [Strand]: 8552..8634 [-]
• Host baterium: Escherichia coli O55:H7 str. RM12579

Cargo genes

• Drug resistance gene: blaTEM-1B
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -