Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100403
Name   oriT_pCV56B in_silico
Organism   Lactococcus lactis subsp. lactis CV56
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_017487 (32692..33154 [+], 463 nt)
oriT length   463 nt
IRs (inverted repeats)      IR1: 138..147, 152..161  (CCCATTTTAT..ATAAAATGGG)
  IR2: 207..219, 223..235  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 242..255, 268..281  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      290..291
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 463 nt

>oriT_pCV56B
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAAGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   474 GenBank   WP_014570908
Name   Relaxase_pCV56B insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47674.28 Da        Isoelectric Point: 8.1241

>WP_014570908.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEARKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   864 GenBank   NC_017487
Plasmid name   pCV56B Incompatibility group   _
Plasmid size   35934 bp Coordinate of oriT [Strand]   32692..33154 [+]
Host baterium   Lactococcus lactis subsp. lactis CV56

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -