Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100401
Name   oriT_pEp5 in_silico
Organism   Erwinia pyrifoliae DSM 12163
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_017389 (4305..4486 [+], 182 nt)
oriT length   182 nt
IRs (inverted repeats)      13..20, 24..31  (TTCGGGGC..GCCCTGAA)
Location of nic site      64..65
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 182 nt

>oriT_pEp5
GTTTTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAAAAGGCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTTCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   472 GenBank   WP_012814659
Name   Nuclease_pEp5 insolico UniProt ID   D2TE66
Length   504 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 504 a.a.        Molecular weight: 55708.97 Da        Isoelectric Point: 9.0830

>WP_012814659.1 nuclease [Erwinia pyrifoliae]
MIVKFHPRGRGGGSGPVDYLLGKDRQRDGASVLQGKPEEVLELIDASAYAKKYTSGVLSFAEKDLAPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTVVN
GRLGLHDPNAPENRRALVTPSALPKAKLEAAETITRGLLAIASSGELKTRQDVTEALESAGFDVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRIQRAREVCKSGTERKREENQRR
HSRPRPAAVLSHEPAHERHGTHGQPDVADHRPGFRPVDRLERGHSVVAGAADTRQLHEHPGTEGRAGEAE
RADMGREISGRQPGAFSGAAGRRESGHELDVRQRQEERHQTGTGVEQHDGAGKTVAERIRAATAGLLEKA
GRVGERLRGMADDVWSYATGERGAERARHGFEQAGAEFERAAAPVVVKLNGIEAHRQQERAIQQQKELEL
ERSQRQRTYDGPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure


Source ID Structure
AlphaFold DB D2TE66


Host bacterium

ID   862 GenBank   NC_017389
Plasmid name   pEp5 Incompatibility group   Col440II
Plasmid size   4960 bp Coordinate of oriT [Strand]   4305..4486 [+]
Host baterium   Erwinia pyrifoliae DSM 12163

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -