Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100399
Name   oriT_pSFxv_4 in_silico
Organism   Shigella flexneri 2002017
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_017321 (208..290 [-], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCATGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pSFxv_4
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   470 GenBank   WP_025715427
Name   PutativerelaxaseofpSFxv_4 insolico UniProt ID   A0A1W2MWW5
Length   521 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 521 a.a.        Molecular weight: 58921.40 Da        Isoelectric Point: 6.3996

>WP_025715427.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Shigella]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAESITRWIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARRICKQGCDIKRDENQRR
YSPVHSFDRGITEKTSGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDTGEPGRN
QEAERESGEAEREDMGRALSRGEQRTFSGVARGDESRNELDDGKRETECRKAGTGVTEHDGAGKPFAERI
RAAATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREER
LIQEREHALFLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure



No available structure.




Host bacterium

ID   860 GenBank   NC_017321
Plasmid name   pSFxv_4 Incompatibility group   ColRNAI
Plasmid size   4042 bp Coordinate of oriT [Strand]   208..290 [-]
Host baterium   Shigella flexneri 2002017

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -