Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100397
Name   oriT_pUUH239.1 in_silico
Organism   Klebsiella pneumoniae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_016979 (4957..5136 [+], 180 nt)
oriT length   180 nt
IRs (inverted repeats)      10..17, 21..28  (TTCGGGGC..GCCCTGAA)
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 180 nt

>oriT_pUUH239.1
TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTATTATGTTGGCATGAGTGCGGTAATCAGGAAAGTGCTTCATGTGACAGAGGAAAATGCAGCTGCGGGCGTAAGGCAGAAAATGGAAGTTCAGAGGATATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   468 GenBank   YP_005352162
Name   Nuclease_pUUH239.1 insolico UniProt ID   G7RUW6
Length   501 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 501 a.a.        Molecular weight: 54206.14 Da        Isoelectric Point: 6.8772

>YP_005352162.1 relaxase/mobilization nuclease protein (plasmid) [Klebsiella pneumoniae]
MIVKFHPRGRGGGAGPVDYCWEKTAARGGDRAAGKTGGSPGAIDASPYAKKYTSGVLSFAEAELPPGQRE
QIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNG
RLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLALASSGELKTREDVTGALTAAGFEVVRTTKNS
ISIADPDGGRNIRLKGAIYEQSFSAGEGLGAAIESAAAEYRRDAESRIQRARAVCKSGTERKREENQRRH
QRPRAGYDRGDAVEPAERAAYGRPDVADHHPGLGTADRHEREHSVVAGAADHRELPDYPGAGADAGHAVG
EEPRRAAVALRGEETVLRQSEPESGSLRGGRELDGTGEEIAHDGTGKTVADRIRAATSGLLAKAGRVGER
LRGMAEDVWSYATGERGAERARHALESAGGELEQAVTAFEPVVQRHEMAVAAERAYEQKQHEKELAETRS
RQRSYDGPTLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(56-236)


  Protein structure


Source ID Structure
AlphaFold DB G7RUW6


Host bacterium

ID   858 GenBank   NC_016979
Plasmid name   pUUH239.1 Incompatibility group   Col440I
Plasmid size   5247 bp Coordinate of oriT [Strand]   4957..5136 [+]
Host baterium   Klebsiella pneumoniae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -