Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100394
Name   oriT_pMR0211 in_silico
Organism   Providencia stuartii
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_016974 (51190..51740 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pMR0211
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   465 GenBank   YP_005351757
Name   TraI_pMR0211 insolico UniProt ID   A0A140NG30
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>YP_005351757.1 type IV conjugative transfer system protein TraI (plasmid) [Providencia stuartii]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A140NG30


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 49324..58414

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS973_RS00355 45355..45588 - 234 WP_001191890 hypothetical protein -
HS973_RS00360 (pMR0211_0044) 45570..46187 - 618 WP_001249395 hypothetical protein -
HS973_RS00365 (pMR0211_0045) 46355..49327 + 2973 WP_001326173 MobH family relaxase -
HS973_RS00370 (pMR0211_0046) 49324..51189 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
HS973_RS00375 51200..51784 + 585 WP_000332868 hypothetical protein -
HS973_RS00380 51741..52370 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
HS973_RS00385 52380..52826 + 447 WP_000122507 hypothetical protein -
HS973_RS00390 (pMR0211_0050) 52836..53213 + 378 WP_000869297 hypothetical protein -
HS973_RS00395 53213..53875 + 663 WP_001231464 hypothetical protein -
HS973_RS00400 54056..54199 + 144 WP_001275801 hypothetical protein -
HS973_RS00405 (pMR0211_0051) 54211..54576 + 366 WP_001052530 hypothetical protein -
HS973_RS00410 54721..55002 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HS973_RS00415 54999..55625 + 627 WP_172684910 TraE/TraK family type IV conjugative transfer system protein traE
HS973_RS00420 (pMR0211_0053) 55609..56526 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HS973_RS00425 (pMR0211_0054) 56526..57839 + 1314 WP_024131605 TraB/VirB10 family protein traB
HS973_RS00430 57836..58414 + 579 WP_172684911 type IV conjugative transfer system lipoprotein TraV traV
HS973_RS00435 58418..58810 + 393 WP_000479535 TraA family conjugative transfer protein -
HS973_RS00440 (pMR0211_0056) 59095..60357 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HS973_RS00445 (pMR0211_0058) 60681..61826 + 1146 WP_014386387 class C beta-lactamase CMY-16 -
HS973_RS00450 (pMR0211_0059) 61920..62453 + 534 WP_001221666 lipocalin family protein -
HS973_RS00455 (pMR0211_0060) 62450..62767 - 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
HS973_RS01075 63024..63389 + 366 Protein_92 LuxR family transcriptional regulator -

Host bacterium

ID   855 GenBank   NC_016974
Plasmid name   pMR0211 Incompatibility group   IncA/C2
Plasmid size   178277 bp Coordinate of oriT [Strand]   51190..51740 [+]
Host baterium   Providencia stuartii

Cargo genes

Drug resistance gene   floR, tet(A), aph(6)-Id, aph(3'')-Ib, sul2, blaCMY-16, ant(2'')-Ia, cmlA1, blaOXA-10, ant(3'')-Ia, qacE, sul1, mph(E), msr(E), armA, blaNDM-1, aph(3')-VI, qnrA1, aac(6')-Ib
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -