Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100385
Name   oriT_pSMA198 in_silico
Organism   Streptococcus macedonicus ACA-DC 198
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_016750 (9085..9548 [+], 464 nt)
oriT length   464 nt
IRs (inverted repeats)      IR1: 137..148, 153..164  (TGCCCATTTTAT..ATCAAATGGGCA)
  IR2: 208..220, 224..236  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 242..256, 269..283  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      291..292
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 464 nt

>oriT_pSMA198
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   456 GenBank   WP_014295531
Name   Relaxase_pSMA198 insolico UniProt ID   H2A9L4
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47704.35 Da        Isoelectric Point: 8.6701

>WP_014295531.1 relaxase/mobilization nuclease domain-containing protein [Streptococcus macedonicus]
MATIAKISNGASAASALNYALGQNRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKR
TKQNEFSAVEQREPALTPLERDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   846 GenBank   NC_016750
Plasmid name   pSMA198 Incompatibility group   _
Plasmid size   12728 bp Coordinate of oriT [Strand]   9085..9548 [+]
Host baterium   Streptococcus macedonicus ACA-DC 198

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -