Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100382 |
Name | oriT_pHK17a |
Organism | Escherichia coli |
Sequence Completeness | intact |
NCBI accession of oriT (coordinates [strand]) | NC_016039 (32807..33085 [-], 279 nt) |
oriT length | 279 nt |
IRs (inverted repeats) | 220..227, 230..237 (GCAAAAAC..GTTTTTGC) |
Location of nic site | 246..247 |
Conserved sequence flanking the nic site |
GTAGTGTGT|GG |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 279 nt
>oriT_pHK17a
CGCTAGCAGCGCCCCTGACGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGCTGTAGCTGCGTTAACGACGCTGCTTTAAAAAAATCAGATTTAAACAATATAAATCCACAAATACAACTCAATGATATTAAAGATAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
CGCTAGCAGCGCCCCTGACGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGCTGTAGCTGCGTTAACGACGCTGCTTTAAAAAAATCAGATTTAAACAATATAAATCCACAAATACAACTCAATGATATTAAAGATAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 453 | GenBank | YP_004870085 |
Name | TraI_pHK17a | UniProt ID | G3G3U1 |
Length | 1756 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191868.57 Da Isoelectric Point: 5.7970
>YP_004870085.1 protein TraI (plasmid) [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHRQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPMTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHRQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPMTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32239..60509
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HS773_RS00185 (PHK17A_p044) | 27556..27870 | + | 315 | WP_000872086 | hypothetical protein | - |
HS773_RS00460 | 28060..28173 | + | 114 | Protein_40 | DUF5431 family protein | - |
HS773_RS00190 | 28118..28240 | + | 123 | WP_223412041 | Hok/Gef family protein | - |
HS773_RS00195 | 28541..28790 | - | 250 | Protein_42 | hypothetical protein | - |
HS773_RS00200 (PHK17A_p049) | 29086..29409 | + | 324 | WP_000533253 | hypothetical protein | - |
HS773_RS00205 (PHK17A_p050) | 29468..29710 | + | 243 | WP_000540588 | hypothetical protein | - |
HS773_RS00465 | 29976..30236 | + | 261 | WP_071600428 | hypothetical protein | - |
HS773_RS00210 (PHK17A_p053) | 30707..31003 | + | 297 | WP_001272251 | hypothetical protein | - |
HS773_RS00215 (PHK17A_p054) | 31113..31934 | + | 822 | WP_001234437 | DUF932 domain-containing protein | - |
HS773_RS00220 (PHK17A_p056) | 32239..32841 | - | 603 | WP_000243715 | transglycosylase SLT domain-containing protein | virB1 |
HS773_RS00225 (PHK17A_p057) | 33162..33545 | + | 384 | WP_001151529 | conjugal transfer relaxosome DNA-binding protein TraM | - |
HS773_RS00230 (PHK17A_p058) | 33677..34384 | + | 708 | WP_227626108 | transcriptional regulator TraJ family protein | - |
HS773_RS00235 (PHK17A_p059) | 34504..34731 | + | 228 | WP_000589556 | conjugal transfer relaxosome protein TraY | - |
HS773_RS00240 (PHK17A_p060) | 34765..35127 | + | 363 | WP_012881131 | type IV conjugative transfer system pilin TraA | - |
HS773_RS00245 (PHK17A_p061) | 35142..35453 | + | 312 | WP_000012100 | type IV conjugative transfer system protein TraL | traL |
HS773_RS00250 (PHK17A_p062) | 35475..36041 | + | 567 | WP_000399780 | type IV conjugative transfer system protein TraE | traE |
HS773_RS00255 (PHK17A_p063) | 36028..36756 | + | 729 | WP_001230769 | type-F conjugative transfer system secretin TraK | traK |
HS773_RS00260 (PHK17A_p064) | 36756..38183 | + | 1428 | WP_014106971 | F-type conjugal transfer pilus assembly protein TraB | traB |
HS773_RS00265 (PHK17A_p065) | 38173..38763 | + | 591 | WP_000002784 | conjugal transfer pilus-stabilizing protein TraP | - |
HS773_RS00270 (PHK17A_p066) | 38750..39070 | + | 321 | WP_012881128 | conjugal transfer protein TrbD | - |
HS773_RS00275 (PHK17A_p067) | 39067..39582 | + | 516 | WP_000809881 | type IV conjugative transfer system lipoprotein TraV | traV |
HS773_RS00280 (PHK17A_p068) | 39717..39938 | + | 222 | WP_001278683 | conjugal transfer protein TraR | - |
HS773_RS00285 (PHK17A_p069) | 39931..40407 | + | 477 | WP_012881127 | hypothetical protein | - |
HS773_RS00290 (PHK17A_p070) | 40506..40724 | + | 219 | WP_000556745 | hypothetical protein | - |
HS773_RS00295 (PHK17A_p071) | 40752..41099 | + | 348 | WP_000836682 | hypothetical protein | - |
HS773_RS00300 (PHK17A_p072) | 41225..43855 | + | 2631 | WP_000069772 | type IV secretion system protein TraC | virb4 |
HS773_RS00305 (PHK17A_p073) | 43852..44238 | + | 387 | WP_000214084 | type-F conjugative transfer system protein TrbI | - |
HS773_RS00310 (PHK17A_p074) | 44235..44867 | + | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
HS773_RS00315 (PHK17A_p075) | 44864..45856 | + | 993 | WP_000830849 | conjugal transfer pilus assembly protein TraU | traU |
HS773_RS00320 (PHK17A_p076) | 45886..46191 | + | 306 | WP_000224418 | hypothetical protein | - |
HS773_RS00325 (PHK17A_p077) | 46200..46838 | + | 639 | WP_012881126 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
HS773_RS00330 (PHK17A_p078) | 46835..48685 | + | 1851 | WP_012881125 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
HS773_RS00335 (PHK17A_p079) | 48712..48969 | + | 258 | WP_000864353 | conjugal transfer protein TrbE | - |
HS773_RS00340 (PHK17A_p080) | 48962..49705 | + | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
HS773_RS00345 (PHK17A_p081) | 49719..50060 | + | 342 | WP_000556795 | conjugal transfer protein TrbA | - |
HS773_RS00350 (PHK17A_p082) | 50041..50376 | - | 336 | WP_000736414 | hypothetical protein | - |
HS773_RS00355 (PHK17A_p083) | 50457..50741 | + | 285 | WP_000624107 | type-F conjugative transfer system pilin chaperone TraQ | - |
HS773_RS00360 (PHK17A_p084) | 50728..51273 | + | 546 | WP_000059830 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
HS773_RS00365 (PHK17A_p085) | 51203..51550 | + | 348 | WP_071840683 | P-type conjugative transfer protein TrbJ | - |
HS773_RS00370 (PHK17A_p086) | 51531..51923 | + | 393 | WP_014106973 | F-type conjugal transfer protein TrbF | - |
HS773_RS00375 (PHK17A_p087) | 51910..53283 | + | 1374 | WP_014106974 | conjugal transfer pilus assembly protein TraH | traH |
HS773_RS00380 (PHK17A_p088) | 53280..56105 | + | 2826 | WP_012881124 | conjugal transfer mating-pair stabilization protein TraG | traG |
HS773_RS00385 (PHK17A_p089) | 56102..56611 | + | 510 | WP_000628111 | conjugal transfer entry exclusion protein TraS | - |
HS773_RS00390 (PHK17A_p090) | 56625..57356 | + | 732 | WP_012881123 | conjugal transfer complement resistance protein TraT | - |
HS773_RS00395 (PHK17A_p091) | 57559..58296 | + | 738 | WP_014106975 | hypothetical protein | - |
HS773_RS00400 (PHK17A_p092) | 58347..60509 | + | 2163 | WP_014106976 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 843 | GenBank | NC_016039 |
Plasmid name | pHK17a | Incompatibility group | IncFII |
Plasmid size | 70060 bp | Coordinate of oriT [Strand] | 32807..33085 [-] |
Host baterium | Escherichia coli |
Cargo genes
Drug resistance gene | blaCTX-M-14 |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |