Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100380
• Name: oriT_pGB2001
• Organism: Streptococcus agalactiae
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_015973 (1010..1048 [-], 39 nt)
• oriT length: 39 nt
• IRs (inverted repeats): 3..10, 14..20 (ACATTTAC..GTAAAGT)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: GCG|TTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 39 nt

>oriT_pGB2001
GCACATTTACGAAGTAAAGTATAGTGCGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_pGB2001

• ID: 451
• GenBank: YP_004831119
• Name: Mob_pGB2001
• UniProt ID: G3ER43
• Length: 500 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 500 a.a.
• Molecular weight: 58463.75 Da
• Isoelectric Point: 6.0256

>YP_004831119.1 plasmid mobilization protein (plasmid) [Streptococcus agalactiae]
MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNYELTDRDRSVSYEKQIKDYVNEKKSSK
RAIRKDAVLCDEWIITSDKDFFERLDQEQTRAFFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVP
MVDGKLSSKAVFTREELKAIQEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGA
PRYWHKEEDRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDKKIEE
YTRIDSEASERLSELSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETTQLLVEQNRNLRKISFQ
DLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMSEVVEKLEQVINQNEVLRKALEGMKQAKE
RAERELLQEEWKTQRLETENQNLRQENKELKVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGY
EPKRSKGRSI

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-194)

Protein structure

• AlphaFold DB: G3ER43

Host bacterium

• ID: 841
• GenBank: NC_015973
• Plasmid name: pGB2001
• Incompatibility group: _
• Plasmid size: 4967 bp
• Coordinate of oriT [Strand]: 1010..1048 [-]
• Host baterium: Streptococcus agalactiae

Cargo genes

• Drug resistance gene: erm(T)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -