Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100379
Name   oriT_pVF22 in_silico
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_015901 (18784..19246 [+], 463 nt)
oriT length   463 nt
IRs (inverted repeats)      IR1: 138..147, 152..161  (CCCATTTTAT..ATAAAATGGG)
  IR2: 207..219, 223..235  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 241..255, 268..282  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      290..291
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 463 nt

>oriT_pVF22
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTCATACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGACTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   450 GenBank   YP_004770047
Name   Mob_pVF22 insolico UniProt ID   G1FE58
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47647.21 Da        Isoelectric Point: 7.6431

>YP_004770047.1 mobilization protein (plasmid) [Lactococcus lactis subsp. lactis bv. diacetylactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB G1FE58


Host bacterium

ID   840 GenBank   NC_015901
Plasmid name   pVF22 Incompatibility group   _
Plasmid size   22166 bp Coordinate of oriT [Strand]   18784..19246 [+]
Host baterium   Lactococcus lactis subsp. lactis bv. diacetylactis

Cargo genes

Drug resistance gene   ClpL, tet(S)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -