Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100378
• Name: oriT_pDRPIS7493
• Organism: Streptococcus pseudopneumoniae IS7493
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_015876 (4283..4315 [+], 33 nt)
• oriT length: 33 nt
• IRs (inverted repeats): 3..10, 14..20 (ACTTTTAC..GTAAAGT)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: GCG|TTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 33 nt

>oriT_pDRPIS7493
GCACTTTTACGAAGTAAAGTATAGTGCGTTATA

oriT secondary structure

Predicted by RNAfold.

Relaxase

PutativerelaxaseofpDRPIS7493

• ID: 449
• GenBank: WP_000121181
• Name: PutativerelaxaseofpDRPIS7493
• UniProt ID: G0ID35
• Length: 500 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 500 a.a.
• Molecular weight: 58405.38 Da
• Isoelectric Point: 6.4144

>WP_000121181.1 MULTISPECIES: MobV family relaxase [Streptococcus]
MSYMVARMQKMKVGNLGGAYKHNERIFEKHSNKDIDTSRSHLNYELTDRDRSVSYERQIKDYVNENKSSK
RGIRKDAVLCDEWIITSDKDFFEKLGQEQTRKFFETAKNYFAENYGETNVAYASVHLDESTPHMHLGIVP
MRNGKLSSKVMFNREELKHIQEDLPKYMNEHGFELQRGKRDSKEQHLSVADYKEKMGREALNQELLDLGA
PKYWHTEERRPATMSEIQDYEDIGSLFADSDVTFNIREATLEERFKWLDTHRNDLRADLGRLEGVLEKKI
DEYGKIDSEASERLSELSEVNSEVNSRQEEIKGLKSDSKRLSDDVVRLERAHREKTQLLVEQSSNLSKIS
YRDLDRRRVAKELQEELENATPKLFGDGFNFTSDFVGRLKTFVSDVVEKLEQAVNQNELLRNALAGMKEA
KSRLENSLSHAEWKNQQLETENKALKLENRELKVSKNLLDDLSEVITEKEVTSLNKRLENLRETRELSRK
RHEPTKGRSI

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-194)

Protein structure

• AlphaFold DB: G0ID35

Host bacterium

• ID: 839
• GenBank: NC_015876
• Plasmid name: pDRPIS7493
• Incompatibility group: _
• Plasmid size: 4727 bp
• Coordinate of oriT [Strand]: 4283..4315 [+]
• Host baterium: Streptococcus pseudopneumoniae IS7493

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -