Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100377 |
| Name | oriT_pIL7  |
| Organism | Lactococcus lactis subsp. lactis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_015864 (27222..27685 [+], 464 nt) |
| oriT length | 464 nt |
| IRs (inverted repeats) | IR1: 139..148, 153..162 (CCCATTTTAT..ATAAAATGGG) IR2: 208..220, 224..236 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 242..256, 269..283 (CACATTGTAATACAA..TTGTATTACAATGTG) |
| Location of nic site | 291..292 |
| Conserved sequence flanking the nic site |
CTTG|CA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 464 nt
>oriT_pIL7
TTAGTGGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTCATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
TTAGTGGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTCATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 448 | GenBank | YP_004761568 |
| Name | MobD_pIL7 |
UniProt ID | G0WKP2 |
| Length | 505 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 505 a.a. Molecular weight: 59156.86 Da Isoelectric Point: 8.3523
>YP_004761568.1 MobD (plasmid) [Lactococcus lactis subsp. lactis]
MTVIYMPKNSNGTVHSSKDLNQLINYVMNPEKTNDFEYISGQNILNIHSACDEMLATRELAIFTGKSPKK
NEIFGYHFVQSFSPDDNLTPAQVHEIGCKTMKEYLGNSAEFIIATHTDKPHLHNHIVLNATNPLTLNKFH
QNKNDLERLKEISDRISKEYGCKIIDRPEQKLGNSHKNYLVYLAKNSYRKEIKNKLEFLMNHSHTWEDFK
TKASALNLKVNDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKKNLKERIEKNTIGYSVEEVVKLWNDKES
IQEKSQEKEISILVEDWQIEKETEKYLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYQD
KKSPENNKIMYGTTVAKNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYH
ETEKQLDKLDTKIVEYVEKNKSLDYVDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI
MTVIYMPKNSNGTVHSSKDLNQLINYVMNPEKTNDFEYISGQNILNIHSACDEMLATRELAIFTGKSPKK
NEIFGYHFVQSFSPDDNLTPAQVHEIGCKTMKEYLGNSAEFIIATHTDKPHLHNHIVLNATNPLTLNKFH
QNKNDLERLKEISDRISKEYGCKIIDRPEQKLGNSHKNYLVYLAKNSYRKEIKNKLEFLMNHSHTWEDFK
TKASALNLKVNDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKKNLKERIEKNTIGYSVEEVVKLWNDKES
IQEKSQEKEISILVEDWQIEKETEKYLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYQD
KKSPENNKIMYGTTVAKNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYH
ETEKQLDKLDTKIVEYVEKNKSLDYVDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G0WKP2 |
Host bacterium
| ID | 838 | GenBank | NC_015864 |
| Plasmid name | pIL7 | Incompatibility group | _ |
| Plasmid size | 28546 bp | Coordinate of oriT [Strand] | 27222..27685 [+] |
| Host baterium | Lactococcus lactis subsp. lactis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA15 |