Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100376
Name   oriT_pIL5 in_silico
Organism   Lactococcus lactis subsp. lactis
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_015863 (20005..20380 [+], 376 nt)
oriT length   376 nt
IRs (inverted repeats)      IR1: 139..148, 153..162  (CCCATTTTAT..ATAAAATGGG)
  IR2: 208..220, 224..236  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 243..256, 269..282  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      291..292
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 376 nt

>oriT_pIL5
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGGGGCGTTTGTGTAATGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   447 GenBank   YP_004761552
Name   Mob_pIL5 insolico UniProt ID   G0WJT9
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47615.29 Da        Isoelectric Point: 7.6156

>YP_004761552.1 mobilization protein (plasmid) [Lactococcus lactis subsp. lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWLILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB G0WJT9


Host bacterium

ID   837 GenBank   NC_015863
Plasmid name   pIL5 Incompatibility group   _
Plasmid size   23395 bp Coordinate of oriT [Strand]   20005..20380 [+]
Host baterium   Lactococcus lactis subsp. lactis

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   cadmium resistance
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -