Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100375
Name   oriT_pLAtc1 in_silico
Organism   Acidithiobacillus caldus SM-1
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_015852 (9153..9462 [-], 310 nt)
oriT length   310 nt
IRs (inverted repeats)      IR1:78..85, 88..95  (CAAGGGGC..GCCCCTTG)
  IR2: 155..161, 165..171  (ACATGGC..GCCATGT)
Location of nic site      191..192
Conserved sequence flanking the
  nic site    		  CATCCTG|T
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 310 nt

>oriT_pLAtc1
CGTGTCTCCTTCTCGCCGGGGCGGGGGCGGGGGTGTGTCTTGGACAGTAAGAAGCGAAGCGCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAAGCGAAGCGCCGAATGGGCAGGCGGGGATTGGCAGGAACATGGCGTAGCCATGTGTATGACAATCCGCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGCTGCACGGGACGCACTTTGCAACAAGCACAAAAGGCGATATGCTGTCAACTCCGCAACCAGTAAGCAAAGGAGTTAGCACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   446 GenBank   WP_014003860
Name   Mob_pLAtc1 insolico UniProt ID   _
Length   843 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 843 a.a.        Molecular weight: 97246.14 Da        Isoelectric Point: 9.5957

>WP_014003860.1 TraI/MobA(P) family conjugative relaxase [Acidithiobacillus caldus]
MPNSKSKHKTASIRDLADYIREPHNQNPDEKVLYANGRGFLCDDHAAQREEMVALAAESVRSKNPVNHYI
MSWREGEQPSPEQVEEAVSIFMEELGWKDHQAIYGLHKDTDNIHLHIAINRVHPETLKIVEINRGFDIEL
AHRAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKLRQPDQKKRDMENRTGEKSALRIAIEDGAAI
IKQAQSWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAQR
EPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAM
RSVIAAEQAAEKAALKEKHQKQREQWRQQFRPYPDLEQWQRMQKSPDLAEQWRHRASEPQRIEGDRSEPP
TPRDIRAYVPEIVGQQVHYSRKDDAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVTGN
DEYKAMCVKLAAEHGFKITNPELQERIQQERQRIQEERAQAMKAEQLKQFERYAEAVGAERYRVTSIKMY
PDGKKQTFILDKKDGVTRGFTPEEIAQRMPEMLRLQRRAENLYYTPLSDKKHHILIDDMDREKLERLIHD
GYRPAVVLESSPGNYQAIITVPKLGTPHDRDVGNRLSEALNREYGDRKLSGAIHPHRAPGFENRKPKHQR
EDGSYPEVRLLKAERRECAKTLELARQIDADYQRQAQERAQKALERAQAVGDREVQPDALKIDSRASNEA
VMGIREKEEGRDWDGYAQRTARYAFGFAGDRQAARLEKYREQWLKLEGRDPQRQQEQAKARKVERDNSPG
MSR

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(436-521)

Relaxase/Mobilisation nuclease domain (Relaxase)

(10-268)

RepB DNA-primase from phage plasmid (RepB_primase)

(542-761)


  Protein structure



No available structure.




Host bacterium

ID   836 GenBank   NC_015852
Plasmid name   pLAtc1 Incompatibility group   IncQ2
Plasmid size   9778 bp Coordinate of oriT [Strand]   9153..9462 [-]
Host baterium   Acidithiobacillus caldus SM-1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -