Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100374
Name   oriT_pS51A in_silico
Organism   Enterobacter cloacae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_015175 (4821..5007 [-], 187 nt)
oriT length   187 nt
IRs (inverted repeats)      15..22, 26..33  (TTCGGGGC..GCCCTGAA)
Location of nic site      66..67
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 187 nt

>oriT_pS51A
GGTTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCATGAGTGCGGTTATCAGGAAAGTGCTTCATGTAGCAGGAGAAAATATGCGGCTGAGACGCGTCAGTGCTGAATGCCGGTTCAGGAGATATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   445 GenBank   YP_004272405
Name   Mob_pS51A insolico UniProt ID   E9RGW1
Length   511 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 511 a.a.        Molecular weight: 55787.21 Da        Isoelectric Point: 7.3939

>YP_004272405.1 mobilization protein A (plasmid) [Enterobacter cloacae]
MRCWKKGGMMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSFA
EAELPPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLAGKRLQPYYDRADRPR
IDAWQTVVNGRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLSLASSGGLKTREDVTGALTAAG
FEVVRTTKNSISIADPDGGRNIRLKGAIYEQSFSAGEGLGAAIESAAAEYRRDAESRIQRARAVCKSGTE
RKREENQRRHQRPRAGYDRGDAVEPPERAAYGRPDMADHHPGLGTADRHEREHSVVAGAADHRELPDYPG
AGADAGHAVGEEPRRAAVALRGEETVLRQSEPESGSVRGGRELDGTGEEIAHDGTGKTVADRIRAATSGL
LAKAGRVGERLRGMAEDVWSYATGERGAERARHALESAGGELEQAVTAFEPVVQRHEMAVAAERAYEQRQ
HEKELAETRSRQRSYNGPTLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(66-246)


  Protein structure


Source ID Structure
AlphaFold DB E9RGW1


Host bacterium

ID   835 GenBank   NC_015175
Plasmid name   pS51A Incompatibility group   Col440II
Plasmid size   5782 bp Coordinate of oriT [Strand]   4821..5007 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -