Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100373
• Name: oriT_p157F-NC2
• Organism: Bifidobacterium longum subsp. infantis 157F
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_015066 (3418..3449 [-], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): 1..8, 12..19 (ATGCAACC..GGTTGCAT)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: TAAGTGCG|CCCT
• Note: predicted by the oriTfinder

oriT sequence

• Length: 32 nt

>oriT_p157F-NC2
ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_p157F-NC2

• ID: 444
• GenBank: WP_013582285
• Name: MobA_p157F-NC2
• UniProt ID: E8MXD7
• Length: 566 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 566 a.a.
• Molecular weight: 64683.13 Da
• Isoelectric Point: 9.3424

>WP_013582285.1 MobA/MobL family protein [Bifidobacterium longum]
MAIYHLSVSNVSRASGSRATATLSYITGKRVHDERRGETYDYGRKERVLRVGTLLPEGASAEFADPAVLF
NAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAATYAIHEDREGNNPHAHILVA
NRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQKTDKRGRRQWKRMSVSLNPLDRKAKLKALRE
SWAKTCNARLDETARIDHRSLEDQGSDLEPTIHEGYAARAIERAGGVSERCEANREIRRSNGLLTAIRTE
LGRIFDRLGELFAAKIRQLRQRQARPEPAREPNWRYFEGDARRQLEADRADHTAAIRGKLMGAKADVRNR
EEWWRSHGTEEFEAAKQIIDVARAASRGARDANVFKRGRLLRNAEQVAGEQSEKLRGAVPWLEDTDIPSD
WDQANRFRMRTTKAIHDHDMQPRTGLVDELERQLEQAERAEGEKPSPEQVKALALRMAEQRERAAEDPTQ
DMNRPEPSGDEPLDLSEPEDLGESGPFEERSAPRTKADLLREIRQRADRRTGQQEAEQRQIDPWQAQPPQ
RRGHGR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (19-253)

Protein structure

• AlphaFold DB: E8MXD7

Host bacterium

• ID: 834
• GenBank: NC_015066
• Plasmid name: p157F-NC2
• Incompatibility group: _
• Plasmid size: 3624 bp
• Coordinate of oriT [Strand]: 3418..3449 [-]
• Host baterium: Bifidobacterium longum subsp. infantis 157F

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -