Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100369 |
| Name | oriT_pCT  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_014477 (32055..32146 [-], 92 nt) |
| oriT length | 92 nt |
| IRs (inverted repeats) | IR1: 7..15, 17..25 (GTCGGGGCT..AGCCCTGAC) IR2: 32..49, 52..69 (GTAATCGTATAGCCGTGC..GCGCGGTTATACGATTAC) |
| Location of nic site | 77..78 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 92 nt
>oriT_pCT
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTTTCAGGC
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTTTCAGGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 440 | GenBank | YP_003864306 |
| Name | Nuclease_pCT |
UniProt ID | E0WGZ0 |
| Length | 906 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 906 a.a. Molecular weight: 104972.66 Da Isoelectric Point: 8.2349
>YP_003864306.1 relaxase/mobilisation nuclease (plasmid) [Escherichia coli]
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIDQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDKAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIDQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDKAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | E0WGZ0 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 37727..85729
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXG14_RS00225 (pCT_053) | 35446..37746 | - | 2301 | WP_013307869 | F-type conjugative transfer protein TrbC | - |
| HXG14_RS00230 (pCT_054) | 37727..38851 | - | 1125 | WP_000152382 | DsbC family protein | trbB |
| HXG14_RS00235 (pCT_055) | 38848..40146 | - | 1299 | WP_001402878 | IncI1-type conjugal transfer protein TrbA | trbA |
| HXG14_RS00530 | 40943..41038 | + | 96 | WP_001363141 | DinQ-like type I toxin DqlB | - |
| HXG14_RS00245 | 41101..41277 | - | 177 | WP_001054907 | hypothetical protein | - |
| HXG14_RS00250 (pCT_057) | 41427..41894 | - | 468 | WP_000598962 | thermonuclease family protein | - |
| HXG14_RS00255 (pCT_058) | 42085..42840 | - | 756 | WP_001282653 | IS21-like element ISSso4 family helper ATPase IstB | - |
| HXG14_RS00260 (pCT_059) | 42857..44392 | - | 1536 | WP_000447015 | IS21 family transposase | - |
| HXG14_RS00265 (pCT_060) | 44698..45321 | + | 624 | WP_001155247 | helix-turn-helix domain-containing protein | - |
| HXG14_RS00270 (pCT_061) | 45519..45734 | - | 216 | WP_000266543 | hypothetical protein | - |
| HXG14_RS00275 (pCT_062) | 45738..46106 | - | 369 | WP_001345852 | hypothetical protein | - |
| HXG14_RS00280 (pCT_064) | 46397..46639 | - | 243 | WP_000650923 | hypothetical protein | - |
| HXG14_RS00285 | 46969..47121 | + | 153 | WP_001302699 | Hok/Gef family protein | - |
| HXG14_RS00290 (pCT_066) | 47259..48845 | - | 1587 | WP_000004564 | TnsA endonuclease N-terminal domain-containing protein | - |
| HXG14_RS00295 (pCT_067) | 49119..49772 | - | 654 | WP_001401768 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HXG14_RS00300 (pCT_068) | 49860..52025 | - | 2166 | WP_000691808 | DotA/TraY family protein | traY |
| HXG14_RS00305 (pCT_069) | 52100..52669 | - | 570 | WP_000650752 | conjugal transfer protein TraX | - |
| HXG14_RS00310 (pCT_070) | 52666..53871 | - | 1206 | WP_001189548 | conjugal transfer protein TraW | traW |
| HXG14_RS00315 (pCT_071) | 53829..54449 | - | 621 | WP_000286854 | hypothetical protein | traV |
| HXG14_RS00320 (pCT_072) | 54449..57493 | - | 3045 | WP_013307872 | conjugal transfer nucleotide-binding protein | traU |
| HXG14_RS00325 (pCT_073) | 57788..58414 | - | 627 | WP_000785703 | hypothetical protein | traT |
| HXG14_RS00535 (pCT_074) | 58521..58772 | - | 252 | WP_001277573 | hypothetical protein | - |
| HXG14_RS00335 (pCT_075) | 58829..59227 | - | 399 | WP_000088813 | DUF6750 family protein | traR |
| HXG14_RS00340 (pCT_076) | 59274..59804 | - | 531 | WP_000987023 | conjugal transfer protein TraQ | traQ |
| HXG14_RS00345 (pCT_077) | 59801..60514 | - | 714 | WP_000787003 | conjugal transfer protein TraP | traP |
| HXG14_RS00350 (pCT_078) | 60511..61848 | - | 1338 | WP_013307873 | conjugal transfer protein TraO | traO |
| HXG14_RS00355 (pCT_079) | 61852..62826 | - | 975 | WP_000547383 | DotH/IcmK family type IV secretion protein | traN |
| HXG14_RS00360 (pCT_080) | 62837..63532 | - | 696 | WP_001681668 | DotI/IcmL family type IV secretion protein | traM |
| HXG14_RS00365 (pCT_081) | 63544..63894 | - | 351 | WP_001056367 | hypothetical protein | traL |
| HXG14_RS00370 (pCT_082) | 63911..67972 | - | 4062 | WP_013307874 | LPD7 domain-containing protein | - |
| HXG14_RS00375 (pCT_083) | 68326..68907 | - | 582 | WP_025750394 | hypothetical protein | - |
| HXG14_RS00380 (pCT_084) | 68915..69370 | - | 456 | Protein_74 | IS5 family transposase | - |
| HXG14_RS00385 (pCT_085) | 69405..70280 | - | 876 | WP_001617865 | extended-spectrum class A beta-lactamase CTX-M-14 | - |
| HXG14_RS00390 (pCT_086) | 70530..71792 | - | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| HXG14_RS00395 (pCT_087) | 72204..72494 | - | 291 | WP_000817801 | IcmT/TraK family protein | traK |
| HXG14_RS00400 (pCT_088) | 72491..73639 | - | 1149 | WP_013307876 | plasmid transfer ATPase TraJ | virB11 |
| HXG14_RS00405 (pCT_089) | 73623..74459 | - | 837 | WP_000745049 | type IV secretory system conjugative DNA transfer family protein | traI |
| HXG14_RS00410 (pCT_090) | 74461..74913 | - | 453 | Protein_80 | DotD/TraH family lipoprotein | - |
| HXG14_RS00415 (pCT_091) | 75017..76219 | - | 1203 | WP_013307879 | conjugal transfer protein TraF | - |
| HXG14_RS00420 (pCT_092) | 76321..77142 | - | 822 | WP_001681675 | hypothetical protein | traE |
| HXG14_RS00425 (pCT_093) | 77355..78479 | - | 1125 | WP_013307880 | site-specific integrase | - |
| HXG14_RS00430 (pCT_094) | 78534..78893 | + | 360 | WP_013307881 | hypothetical protein | - |
| HXG14_RS00435 (pCT_095) | 78898..79194 | - | 297 | WP_001014414 | phage tail protein | - |
| HXG14_RS00440 (pCT_096) | 79373..79528 | + | 156 | WP_001358489 | hypothetical protein | - |
| HXG14_RS00445 (pCT_097) | 79525..79917 | - | 393 | WP_236918886 | shufflon protein C | - |
| HXG14_RS00450 (pCT_100) | 80072..81337 | - | 1266 | WP_013307882 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HXG14_RS00455 (pCT_102) | 81355..81981 | - | 627 | WP_013307883 | prepilin peptidase | - |
| HXG14_RS00460 (pCT_103) | 81997..82482 | - | 486 | WP_001080767 | lytic transglycosylase domain-containing protein | virB1 |
| HXG14_RS00465 (pCT_104) | 82527..83063 | - | 537 | WP_000111521 | type 4 pilus major pilin | - |
| HXG14_RS00470 (pCT_105) | 83125..84219 | - | 1095 | WP_000697154 | type II secretion system F family protein | - |
| HXG14_RS00475 (pCT_106) | 84221..85729 | - | 1509 | WP_000880806 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HXG14_RS00480 (pCT_107) | 85832..86290 | - | 459 | WP_000095887 | type IV pilus biogenesis protein PilP | - |
| HXG14_RS00485 (pCT_108) | 86280..87575 | - | 1296 | WP_000129885 | type 4b pilus protein PilO2 | - |
| HXG14_RS00490 (pCT_109) | 87596..89215 | - | 1620 | WP_001035562 | PilN family type IVB pilus formation outer membrane protein | - |
| HXG14_RS00495 (pCT_110) | 89247..89660 | - | 414 | WP_124983997 | type IV pilus biogenesis protein PilM | - |
Host bacterium
| ID | 830 | GenBank | NC_014477 |
| Plasmid name | pCT | Incompatibility group | IncB/O/K/Z |
| Plasmid size | 93629 bp | Coordinate of oriT [Strand] | 32055..32146 [-] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | blaCTX-M-14 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |