Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100363
• Name: oriT_p75
• Organism: Escherichia coli ETEC 1392/75
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_014235 (3147..3231 [-], 85 nt)
• oriT length: 85 nt
• IRs (inverted repeats): _
• Location of nic site: 58..59
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 85 nt

>oriT_p75
GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCAGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_p75

• ID: 434
• GenBank: YP_003717768
• Name: Mob_p75
• UniProt ID: D7GKX0
• Length: 521 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 521 a.a.
• Molecular weight: 58027.58 Da
• Isoelectric Point: 6.8431

>YP_003717768.1 plasmid mobilisation protein (plasmid) [Escherichia coli ETEC 1392/75]
MERGMIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKVLP
PGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQ
TLVNHHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVR
VTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDLKRDE
NQRRYSPVHNVDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGE
PGRNQDTGRNIAETEREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERI
RATATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDER
LIQEREHALSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (63-241)

Protein structure

• AlphaFold DB: D7GKX0

Host bacterium

• ID: 824
• GenBank: NC_014235
• Plasmid name: p75
• Incompatibility group: ColRNAI
• Plasmid size: 7497 bp
• Coordinate of oriT [Strand]: 3147..3231 [-]
• Host baterium: Escherichia coli ETEC 1392/75

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -