Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100361
Name   oriT_p1081 in_silico
Organism   Escherichia coli ETEC 1392/75
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_014232 (12245..12786 [-], 542 nt)
oriT length   542 nt
IRs (inverted repeats)      243..255, 257..269  (TATTTAATAATTC..GAATTATAAAATA)
 311..318, 321..328  (GCAAAAAC..GTTTTTGC)
Location of nic site      337..338
Conserved sequence flanking the
  nic site    		  GTAGTGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 542 nt

>oriT_p1081
TTACTCTGGCCATAAGATAAAACCTTTCATCATTAAGCAATGAACTTTTCACTATAAATATATATATAGTGTTTACAAGTAAGAAAGACACTCCTAGTAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATAAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCTACCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   432 GenBank   YP_003717559
Name   TraI_p1081 insolico UniProt ID   D7GK71
Length   719 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 719 a.a.        Molecular weight: 78529.35 Da        Isoelectric Point: 7.0600

>YP_003717559.1 putative TraI DNA helicase I (plasmid) [Escherichia coli ETEC 1392/75]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDIFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTLAPGSASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLVRTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTASYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQSIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVGAFVRHQLRHCRAADAGSQPPAVPGG
RQSGTSGAGRLPAGRDSGA

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

TrwC relaxase (TrwC)

(10-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-676)


  Protein structure


Source ID Structure
AlphaFold DB D7GK71


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11849..26204

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXG18_RS00050 (ETEC1392/75_p1018_016) 7376..7810 + 435 WP_000845954 conjugation system SOS inhibitor PsiB -
HXG18_RS00055 (ETEC1392/75_p1018_017) 7807..8566 + 760 Protein_11 plasmid SOS inhibition protein A -
HXG18_RS00605 8739..8888 + 150 Protein_12 plasmid maintenance protein Mok -
HXG18_RS00060 8830..8955 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
HXG18_RS00065 (ETEC1392/75_p1018_018) 9193..9553 - 361 Protein_14 hypothetical protein -
HXG18_RS00070 (ETEC1392/75_p1018_021) 9870..10172 + 303 WP_001272235 hypothetical protein -
HXG18_RS00075 (ETEC1392/75_p1018_022) 10328..10615 + 288 WP_000107522 hypothetical protein -
HXG18_RS00080 (ETEC1392/75_p1018_023) 10732..11553 + 822 WP_001393327 DUF932 domain-containing protein -
HXG18_RS00085 (ETEC1392/75_p1018_024) 11849..12451 - 603 WP_013188482 transglycosylase SLT domain-containing protein virB1
HXG18_RS00090 (ETEC1392/75_p1018_025) 12774..13157 + 384 WP_000124827 conjugal transfer relaxosome DNA-binding protein TraM -
HXG18_RS00095 (ETEC1392/75_p1018_026) 13351..14022 + 672 WP_000283565 conjugal transfer transcriptional regulator TraJ -
HXG18_RS00100 (ETEC1392/75_p1018_027) 14159..14386 + 228 WP_000089263 conjugal transfer relaxosome protein TraY -
HXG18_RS00105 (ETEC1392/75_p1018_028) 14419..14781 + 363 WP_001098998 type IV conjugative transfer system pilin TraA -
HXG18_RS00110 (ETEC1392/75_p1018_029) 14786..15097 + 312 WP_000012113 type IV conjugative transfer system protein TraL traL
HXG18_RS00115 (ETEC1392/75_p1018_030) 15119..15685 + 567 WP_000399782 type IV conjugative transfer system protein TraE traE
HXG18_RS00120 (ETEC1392/75_p1018_031) 15672..16400 + 729 WP_001230775 type-F conjugative transfer system secretin TraK traK
HXG18_RS00125 (ETEC1392/75_p1018_032) 16553..16882 + 330 Protein_26 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
HXG18_RS00130 (ETEC1392/75_p1018_033) 16872..17186 + 315 WP_001417954 P-type conjugative transfer protein TrbJ -
HXG18_RS00135 (ETEC1392/75_p1018_034) 17140..17532 + 393 WP_000138370 F-type conjugal transfer protein TrbF -
HXG18_RS00140 (ETEC1392/75_p1018_035) 17519..18892 + 1374 WP_001393322 conjugal transfer pilus assembly protein TraH traH
HXG18_RS00145 (ETEC1392/75_p1018_036) 18889..21705 + 2817 WP_001395538 conjugal transfer mating-pair stabilization protein TraG traG
HXG18_RS00150 (ETEC1392/75_p1018_037) 21738..22259 + 522 WP_013188483 conjugal transfer entry exclusion protein TraS -
HXG18_RS00155 (ETEC1392/75_p1018_038) 22284..23015 + 732 WP_001366079 conjugal transfer complement resistance protein TraT -
HXG18_RS00160 (ETEC1392/75_p1018_039) 23218..23955 + 738 WP_000199908 hypothetical protein -
HXG18_RS00165 (ETEC1392/75_p1018_040) 24006..26204 + 2199 WP_013188486 type IV conjugative transfer system coupling protein TraD virb4
HXG18_RS00170 (ETEC1392/75_p1018_041) 26204..28225 + 2022 Protein_35 MobF family relaxase -
HXG18_RS00175 (ETEC1392/75_p1018_042) 28224..28529 + 306 Protein_36 type-F conjugative transfer system pilin acetylase TraX -
HXG18_RS00180 (ETEC1392/75_p1018_043) 28588..29448 + 861 WP_000704511 alpha/beta hydrolase -
HXG18_RS00185 (ETEC1392/75_p1018_044) 29551..30111 + 561 WP_000840475 fertility inhibition protein FinO -
HXG18_RS00190 (ETEC1392/75_p1018_045) 30242..30451 + 210 WP_001297500 hypothetical protein -

Host bacterium

ID   822 GenBank   NC_014232
Plasmid name   p1081 Incompatibility group   IncFIC(FII)
Plasmid size   101857 bp Coordinate of oriT [Strand]   12245..12786 [-]
Host baterium   Escherichia coli ETEC 1392/75

Cargo genes

Drug resistance gene   -
Virulence gene   Enterotoxigenic E. coli (ETEC) virulence
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -