Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100358
• Name: oriT_pKKS627
• Organism: Staphylococcus aureus subsp. aureus ST398
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_014156 (4704..4742 [+], 39 nt)
• oriT length: 39 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTG|TTT
• Note: predicted by the oriTfinder

oriT sequence

• Length: 39 nt

>oriT_pKKS627
CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_pKKS627

• ID: 429
• GenBank: YP_003644923
• Name: Mob_pKKS627
• UniProt ID: D6C798
• Length: 420 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>YP_003644923.1 plasmid recombination and mobilization protein (plasmid) [Staphylococcus aureus subsp. aureus ST398]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: D6C798

Host bacterium

• ID: 819
• GenBank: NC_014156
• Plasmid name: pKKS627
• Incompatibility group: _
• Plasmid size: 6242 bp
• Coordinate of oriT [Strand]: 4704..4742 [+]
• Host baterium: Staphylococcus aureus subsp. aureus ST398

Cargo genes

• Drug resistance gene: tet(L), dfrK
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -