Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100350
• Name: oriT_SAP108B
• Organism: Staphylococcus epidermidis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_013390 (1956..2193 [+], 238 nt)
• oriT length: 238 nt
• IRs (inverted repeats): IR1: 52..59, 61..68 (TTGGGGAT..ATCCCCAA) IR2: 94..98, 104..108 (TGGCT..AGCCA)
• Location of nic site: 115..116
• Conserved sequence flanking the nic site: TGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 238 nt

>oriT_SAP108B
AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATTATCCCCAACAAGCAGGCGACGCTACCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTTAACGACTTGATGAATCTTACTTTATCATATAAAGTAAGATGTAAGAAAGGCGTTATCTTTCGAAGAAAGCGTGGTGGGATGTATGAGCGAACAAAATACATTTGTGGCTAGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rlx_SAP108B

• ID: 421
• GenBank: YP_006939270
• Name: Rlx_SAP108B
• UniProt ID: D2JCJ6
• Length: 335 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 335 a.a.
• Molecular weight: 38604.32 Da
• Isoelectric Point: 6.8878

>YP_006939270.1 rlx protein, putative (plasmid) [Staphylococcus epidermidis]
MATTKISATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPDEVTSE
QCNQLGLELAEKLAPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRNVRDFNDEVCRSHSL
SVPDKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFQMHLKQHGVEIERVTPKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERQIQRQQQERESDTDFQPKRPTSNRDESTERDTGVTQSDW
DKFAQDTNELERRRQAAESARLADEKARRDREERERAKQASRRIINNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: D2JCJ6

Host bacterium

• ID: 811
• GenBank: NC_013390
• Plasmid name: SAP108B
• Incompatibility group: _
• Plasmid size: 6206 bp
• Coordinate of oriT [Strand]: 1956..2193 [+]
• Host baterium: Staphylococcus epidermidis

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -