Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100349
• Name: oriT_SAP018B
• Organism: Staphylococcus sp. CDC25
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_013388 (7429..7667 [+], 239 nt)
• oriT length: 239 nt
• IRs (inverted repeats): IR1: 53..60, 62..69 (TTGGGGAT..ATCCCCAA) IR2: 95..99, 105..109 (TGGCT..AGCCA)
• Location of nic site: 116..117
• Conserved sequence flanking the nic site: TGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 239 nt

>oriT_SAP018B
AGTGGCTAGCATTTTGCCACTCATTTTTTGCTTTAGCAAAAACATAAGGGGCTTGGGGATTATCCCCAACAAGCCGGCGTCGCTACCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTTTATTACTTGATGAAATTTAGATTATCATATAATCTAAATTTATATTAAGTAATATCTTTCGTAGAAAGCGTGGTGGGATTTGATGAGCGAACAAAACCATTTTGTGGCTAGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rlx_SAP018B

• ID: 420
• GenBank: YP_006939231
• Name: Rlx_SAP018B
• UniProt ID: D2J6Q7
• Length: 335 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 335 a.a.
• Molecular weight: 38505.25 Da
• Isoelectric Point: 6.5119

>YP_006939231.1 rlx protein, putative (plasmid) [Staphylococcus sp. CDC25]
MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKVTRELYGKTGGNEGHVIIQSFKPGEVTPE
QCNQLGLALAEKFAPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRDLRDFNDDVCREHSL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSKATNMDEFKDHLSQYGIEIARVTPKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERQIQRQQQEREDDNDIQPKRPTSNRDEPTQRDTGVTQSDW
DKFAQDTNELERRRQAAESARLADEKARRDREERERAKQASRTIIDNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: D2J6Q7

Host bacterium

• ID: 810
• GenBank: NC_013388
• Plasmid name: SAP018B
• Incompatibility group: _
• Plasmid size: 8226 bp
• Coordinate of oriT [Strand]: 7429..7667 [+]
• Host baterium: Staphylococcus sp. CDC25

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -