Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100348
Name   oriT_SAP018A in_silico
Organism   Staphylococcus sp. CDC25
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_013387 (31729..31965 [-], 237 nt)
oriT length   237 nt
IRs (inverted repeats)      IR1: 52..59, 61..68  (TTGGGGAT..ATCCCCAA)
  IR2: 94..98, 104..108  (TGGCT..AGCCA)
Location of nic site      115..116
Conserved sequence flanking the
  nic site    		  TGCTTG|CCA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 237 nt

>oriT_SAP018A
TGGCTAGCATTTAAATCACTCATTTTTTATGTAAGAAGAAACATAAGGGGCTTGGGGATTATCCCCAACAAGCAGGCGACACTACCACGTAAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTATATTACTTGATGAAATTTAGATTATCATATAATCTAAATTTATATTAAGTAATATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAATCATTTTGTGGCTAGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   419 GenBank   YP_006939229
Name   Rlx_SAP018A insolico UniProt ID   D2J6Q5
Length   335 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 335 a.a.        Molecular weight: 38532.23 Da        Isoelectric Point: 6.0817

>YP_006939229.1 rlx protein, putative (plasmid) [Staphylococcus sp. CDC25]
MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKVTRELYGKTGGNEGHVIIQSFKPGEVTPE
QCNQLGLALAEKFAPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRDLRDFNDDVCREHSL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSKATNMDEFKDHLSQYGIEIARVTPKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERQIQRQQQEREDDNDIQPKRPTSNRDEPTQRDTGVTQSDW
DKFAQDTNELERRRQAAESARLADEKARRDREERERAEQASRTIIDNDRDYGLEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-242)


  Protein structure


Source ID Structure
AlphaFold DB D2J6Q5


Host bacterium

ID   809 GenBank   NC_013387
Plasmid name   SAP018A Incompatibility group   _
Plasmid size   32487 bp Coordinate of oriT [Strand]   31729..31965 [-]
Host baterium   Staphylococcus sp. CDC25

Cargo genes

Drug resistance gene   aac(6')-aph(2''), blaZ
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21