Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100346
Name   oriT_SAP016A in_silico
Organism   Staphylococcus epidermidis
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_013372 (23350..23587 [+], 238 nt)
oriT length   238 nt
IRs (inverted repeats)      IR1: 53..60, 62..69  (TTGGGGAT..ATCCCCAA)
  IR2: 95..99, 105..109  (TGGCT..AGCCA)
Location of nic site      116..117
Conserved sequence flanking the
  nic site    		  TGCTTG|CCA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 238 nt

>oriT_SAP016A
AGTGGCTAGCATTTTGCCACTCATTTTTTGCTTTAGCAAAAACATAAGGGGCTTGGGGATTATCCCCAACAAGCCGGCGTCGCTACCACGTTAGTGGCTAGCAAAGCCAGTGCTTGCCAAATCACTTTATTACTTGATGAAATTTAGATTATCATATAATCTAAATTTATATTAAGTAATATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAAACATTTTGTGGCTAGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   417 GenBank   YP_006938839
Name   Mob_SAP016A insolico UniProt ID   D2J6F0
Length   335 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 335 a.a.        Molecular weight: 38501.21 Da        Isoelectric Point: 6.2584

>YP_006938839.1 Mobilization protein (plasmid) [Staphylococcus epidermidis]
MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKVTRELYGKTGGNEGHVIIQSFKPGEVTPE
QCNQLGLALAEKFAPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRDLRDFNDNVCREHGL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSKATNMDEFKDHLSQYGIEIARVTPKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERQIQRQQQEREDDNDIQPKRPTSNRDEPTQRDTGVTQSDW
DKFAQDTNELERRRQAAESARLADEKARRDREERERAEQASRTIIDNDRDYGLEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-242)


  Protein structure


Source ID Structure
AlphaFold DB D2J6F0


Host bacterium

ID   807 GenBank   NC_013372
Plasmid name   SAP016A Incompatibility group   _
Plasmid size   43807 bp Coordinate of oriT [Strand]   23350..23587 [+]
Host baterium   Staphylococcus epidermidis

Cargo genes

Drug resistance gene   resistance to teicoplanin and bleomycin
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -