Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100345
• Name: oriT_pO111_5
• Organism: Escherichia coli O111:H- str. 11128
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_013368 (6510..6586 [-], 77 nt)
• oriT length: 77 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 77 nt

>oriT_pO111_5
GGGTGTCGGGGCGCAGCCATGACCCAGTCACATAGCGATAGCGGAGTGTATACTGGCTTAATCATATGGCATCAGTG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pO111_5

• ID: 416
• GenBank: WP_000635823
• Name: Nuclease_pO111_5
• UniProt ID: C8URK3
• Length: 532 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 532 a.a.
• Molecular weight: 59240.91 Da
• Isoelectric Point: 8.4509

>WP_000635823.1 MULTISPECIES: nuclease [Gammaproteobacteria]
MIVKFHARGKGGGSGPVDYLLGRERSREGARVLRGAPEEVRELIDATPFAKKYTSGVLSFAEQTLPPGER
ERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVN
HHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGVEALYHAGALKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGDRLREETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRR
YQRTHSSGERTTGDAEQCRERAVQRHRERTCPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGVDSV
AGKPYCGEHPGYQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGGKQLDGGEEERSKTGQGVSEYD
RAGNALTERLRAIAAGLYAAAERLGERLHGFAAYVRADTTGERATEGTVRRLESAGAEISRAGGPLESVI
YHEQQIRDEQERLARLERERARSWHEEQEREVRVWRGPSMGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (58-239)

Protein structure

No available structure.

Host bacterium

• ID: 806
• GenBank: NC_013368
• Plasmid name: pO111_5
• Incompatibility group: ColRNAI
• Plasmid size: 6673 bp
• Coordinate of oriT [Strand]: 6510..6586 [-]
• Host baterium: Escherichia coli O111:H- str. 11128

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -