Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100344
• Name: oriT_pO26_3
• Organism: Escherichia coli O26:H11 str. 11368
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_013363 (5519..5601 [-], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_pO26_3
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTACTTATTCGGCATCAGGGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pO26_3

• ID: 415
• GenBank: WP_000635796
• Name: Nuclease_pO26_3
• UniProt ID: _
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57810.31 Da
• Isoelectric Point: 6.0801

>WP_000635796.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLPDNLPKTKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGIEVVRVTRS
SISIAGPNGGRNIKLKGAFYEQSFTDGRGVREKAERESRIYRENAEQRVQEARRVCQRGCDLKRDENQRR
YSPVHSLDREIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYSEWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

No available structure.

Host bacterium

• ID: 805
• GenBank: NC_013363
• Plasmid name: pO26_3
• Incompatibility group: ColRNAI
• Plasmid size: 5686 bp
• Coordinate of oriT [Strand]: 5519..5601 [-]
• Host baterium: Escherichia coli O26:H11 str. 11368

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -