Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100343 |
Name | oriT_pO26_2 |
Organism | Escherichia coli O26:H11 str. 11368 |
Sequence Completeness | intact |
NCBI accession of oriT (coordinates [strand]) | NC_013362 (24230..24503 [-], 274 nt) |
oriT length | 274 nt |
IRs (inverted repeats) | 220..227, 230..237 (GCAAAAAC..GTTTTTGC) |
Location of nic site | 246..247 |
Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 274 nt
>oriT_pO26_2
CGCTAGCAGCGCCCCTGACGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGCTGTAGCTGCGTTAACGACGCTGCTTTAAATAAATCAGATTTAAACAATATAAATCCACAAATACAACTCAATGATATTAAAGATAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAAC
CGCTAGCAGCGCCCCTGACGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGCTGTAGCTGCGTTAACGACGCTGCTTTAAATAAATCAGATTTAAACAATATAAATCCACAAATACAACTCAATGATATTAAAGATAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 414 | GenBank | WP_000987009 |
Name | TraI_pO26_2 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192056.90 Da Isoelectric Point: 6.0023
>WP_000987009.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSYDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESGRPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVL
EPKADREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSYDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESGRPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVL
EPKADREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26563..52629
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
ECO26_RS35575 | 21664..21836 | - | 173 | Protein_34 | hypothetical protein | - |
ECO26_RS34950 | 21906..22112 | + | 207 | WP_000547944 | hypothetical protein | - |
ECO26_RS30330 (ECO26_p2-35) | 22136..22432 | + | 297 | WP_001272241 | hypothetical protein | - |
ECO26_RS30335 (ECO26_p2-36) | 22538..23359 | + | 822 | WP_001234501 | DUF932 domain-containing protein | - |
ECO26_RS30340 (ECO26_p2-37) | 23657..24226 | - | 570 | Protein_38 | transglycosylase SLT domain-containing protein | - |
ECO26_RS30345 (ECO26_p2-38) | 24580..24963 | + | 384 | WP_001151530 | conjugal transfer relaxosome DNA-binding protein TraM | - |
ECO26_RS30350 (ECO26_p2-39) | 25155..25802 | + | 648 | WP_000338011 | transcriptional regulator TraJ family protein | - |
ECO26_RS30355 (ECO26_p2-40) | 25922..26149 | + | 228 | WP_000589558 | conjugal transfer relaxosome protein TraY | - |
ECO26_RS30360 (ECO26_p2-41) | 26183..26548 | + | 366 | WP_000340274 | type IV conjugative transfer system pilin TraA | - |
ECO26_RS30365 (ECO26_p2-42) | 26563..26874 | + | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
ECO26_RS30370 (ECO26_p2-43) | 26896..27462 | + | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
ECO26_RS30375 (ECO26_p2-44) | 27449..28177 | + | 729 | WP_001230787 | type-F conjugative transfer system secretin TraK | traK |
ECO26_RS30380 (ECO26_p2-45) | 28177..29604 | + | 1428 | WP_000146635 | F-type conjugal transfer pilus assembly protein TraB | traB |
ECO26_RS30385 (ECO26_p2-46) | 29594..30181 | + | 588 | WP_000002773 | conjugal transfer pilus-stabilizing protein TraP | - |
ECO26_RS30390 (ECO26_p2-47) | 30168..30488 | + | 321 | WP_001057290 | conjugal transfer protein TrbD | virb4 |
ECO26_RS30395 (ECO26_p2-48) | 30481..30732 | + | 252 | WP_001038338 | conjugal transfer protein TrbG | - |
ECO26_RS30400 (ECO26_p2-49) | 30729..31244 | + | 516 | WP_000809890 | type IV conjugative transfer system lipoprotein TraV | traV |
ECO26_RS33555 (ECO26_p2-50) | 31379..31600 | + | 222 | WP_001278700 | conjugal transfer protein TraR | - |
ECO26_RS30410 (ECO26_p2-51) | 31593..32069 | + | 477 | WP_000549578 | hypothetical protein | - |
ECO26_RS30415 (ECO26_p2-52) | 32149..32391 | + | 243 | WP_000556753 | hypothetical protein | - |
ECO26_RS34580 (ECO26_p2-53) | 32393..32740 | + | 348 | WP_000802782 | hypothetical protein | - |
ECO26_RS30425 (ECO26_p2-54) | 32865..35495 | + | 2631 | WP_000069747 | type IV secretion system protein TraC | virb4 |
ECO26_RS30430 (ECO26_p2-55) | 35492..35878 | + | 387 | WP_000214096 | type-F conjugative transfer system protein TrbI | - |
ECO26_RS30435 (ECO26_p2-56) | 35875..36507 | + | 633 | WP_001203735 | type-F conjugative transfer system protein TraW | traW |
ECO26_RS30440 (ECO26_p2-57) | 36504..37496 | + | 993 | WP_000830838 | conjugal transfer pilus assembly protein TraU | traU |
ECO26_RS30445 (ECO26_p2-58) | 37505..38143 | + | 639 | WP_000777701 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
ECO26_RS30450 (ECO26_p2-59) | 38140..38511 | + | 372 | WP_000056336 | hypothetical protein | - |
ECO26_RS30455 (ECO26_p2-60) | 38537..38959 | + | 423 | WP_001229309 | HNH endonuclease signature motif containing protein | - |
ECO26_RS30460 (ECO26_p2-61) | 38956..40806 | + | 1851 | WP_000826170 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
ECO26_RS30465 (ECO26_p2-62) | 40833..41090 | + | 258 | WP_000864325 | conjugal transfer protein TrbE | - |
ECO26_RS30470 (ECO26_p2-63) | 41083..41826 | + | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
ECO26_RS30475 (ECO26_p2-64) | 41987..42223 | + | 237 | WP_000270010 | type-F conjugative transfer system pilin chaperone TraQ | - |
ECO26_RS30480 (ECO26_p2-65) | 42210..42755 | + | 546 | WP_000059817 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
ECO26_RS33565 (ECO26_p2-66) | 42745..43059 | + | 315 | WP_001384255 | P-type conjugative transfer protein TrbJ | - |
ECO26_RS30485 (ECO26_p2-67) | 43013..43405 | + | 393 | WP_000126338 | F-type conjugal transfer protein TrbF | - |
ECO26_RS30490 (ECO26_p2-68) | 43392..43565 | + | 174 | Protein_69 | conjugal transfer protein TraH | - |
ECO26_RS30495 (ECO26_p2-69) | 43592..43984 | + | 393 | WP_000660703 | F-type conjugal transfer protein TrbF | - |
ECO26_RS30500 (ECO26_p2-70) | 43971..45344 | + | 1374 | WP_001137363 | conjugal transfer pilus assembly protein TraH | traH |
ECO26_RS30505 (ECO26_p2-71) | 45341..48160 | + | 2820 | WP_001007051 | conjugal transfer mating-pair stabilization protein TraG | traG |
ECO26_RS30510 (ECO26_p2-72) | 48179..48676 | + | 498 | WP_000605862 | entry exclusion protein | - |
ECO26_RS30515 (ECO26_p2-73) | 48709..49440 | + | 732 | WP_000850424 | conjugal transfer complement resistance protein TraT | - |
ECO26_RS30520 (ECO26_p2-74) | 49643..50380 | + | 738 | WP_000199911 | hypothetical protein | - |
ECO26_RS30525 (ECO26_p2-75) | 50431..52629 | + | 2199 | WP_000009352 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 804 | GenBank | NC_013362 |
Plasmid name | pO26_2 | Incompatibility group | IncFIC(FII) |
Plasmid size | 63365 bp | Coordinate of oriT [Strand] | 24230..24503 [-] |
Host baterium | Escherichia coli O26:H11 str. 11368 |
Cargo genes
Drug resistance gene | aph(3')-Ia |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |