Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100341 |
| Name | oriT_SAP082A  |
| Organism | Staphylococcus aureus |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_013344 (20768..20803 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 31..32 |
| Conserved sequence flanking the nic site |
TAAGTGCGCC|CT |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 36 nt
>oriT_SAP082A
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 412 | GenBank | YP_006938590 |
| Name | Nickase_SAP082A |
UniProt ID | O87361 |
| Length | 665 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>YP_006938590.1 oriT nickase (plasmid) [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| PDB | 4HTE | |
| AlphaFold DB | O87361 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1075..12296
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HHF56_RS00010 (SAP082A_001) | 666..1058 | - | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| HHF56_RS00015 (SAP082A_002) | 1075..1992 | - | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| HHF56_RS00020 (SAP082A_003) | 2070..3710 | - | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HHF56_RS00025 (SAP082A_004) | 3707..4171 | - | 465 | WP_000715273 | hypothetical protein | trsJ |
| HHF56_RS00030 (SAP082A_005) | 4187..6289 | - | 2103 | WP_001094109 | DNA topoisomerase III | - |
| HHF56_RS00035 (SAP082A_006) | 6305..6790 | - | 486 | WP_000735569 | TrsH/TraH family protein | - |
| HHF56_RS00040 (SAP082A_007) | 6800..7876 | - | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| HHF56_RS00045 (SAP082A_008) | 7894..9174 | - | 1281 | WP_000702361 | membrane protein | trsF |
| HHF56_RS00050 (SAP082A_009) | 9186..11204 | - | 2019 | WP_001795400 | DUF87 domain-containing protein | virb4 |
| HHF56_RS00055 (SAP082A_010) | 11219..11902 | - | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| HHF56_RS00060 (SAP082A_011) | 11889..12296 | - | 408 | WP_000110157 | hypothetical protein | trsC |
| HHF56_RS00065 (SAP082A_012) | 12356..12673 | - | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| HHF56_RS00070 (SAP082A_013) | 12690..13664 | - | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| HHF56_RS00075 (SAP082A_014) | 13836..14024 | + | 189 | WP_001063224 | hypothetical protein | - |
| HHF56_RS00080 (SAP082A_015) | 14133..14807 | - | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| HHF56_RS00085 (SAP082A_016) | 14851..14997 | + | 147 | WP_001817822 | hypothetical protein | - |
| HHF56_RS00090 (SAP082A_017) | 15157..15927 | + | 771 | WP_001795128 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | - |
| HHF56_RS00095 (SAP082A_018) | 16037..16711 | - | 675 | WP_001106022 | IS6-like element IS257 family transposase | - |
Host bacterium
| ID | 802 | GenBank | NC_013344 |
| Plasmid name | SAP082A | Incompatibility group | _ |
| Plasmid size | 44116 bp | Coordinate of oriT [Strand] | 20768..20803 [-] |
| Host baterium | Staphylococcus aureus |
Cargo genes
| Drug resistance gene | aadD, aac(6')-aph(2''), mupA, qacD |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |