Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100340 |
| Name | oriT_SAP080A  |
| Organism | Staphylococcus aureus |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_013343 (36546..36581 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 31..32 |
| Conserved sequence flanking the nic site |
TAAGTGCGCC|CT |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 36 nt
>oriT_SAP080A
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 411 | GenBank | YP_006938553 |
| Name | Nickase_SAP080A |
UniProt ID | O87361 |
| Length | 665 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>YP_006938553.1 oriT nickase (plasmid) [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| PDB | 4HTE | |
| AlphaFold DB | O87361 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4596..15817
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HHF57_RS00005 | 14..325 | + | 312 | WP_168445456 | hypothetical protein | - |
| HHF57_RS00010 (SAP080A_001) | 344..748 | - | 405 | WP_001242578 | bleomycin binding protein | - |
| HHF57_RS00015 (SAP080A_002) | 965..1735 | - | 771 | WP_001795128 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | - |
| HHF57_RS00020 (SAP080A_003) | 1895..2041 | - | 147 | WP_001817822 | hypothetical protein | - |
| HHF57_RS00025 (SAP080A_004) | 2085..2759 | + | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| HHF57_RS00030 (SAP080A_005) | 2868..3056 | - | 189 | WP_001063224 | hypothetical protein | - |
| HHF57_RS00035 (SAP080A_006) | 3228..4202 | + | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| HHF57_RS00040 (SAP080A_007) | 4219..4536 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| HHF57_RS00045 (SAP080A_008) | 4596..5003 | + | 408 | WP_000110157 | hypothetical protein | trsC |
| HHF57_RS00050 (SAP080A_009) | 4990..5673 | + | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| HHF57_RS00055 (SAP080A_010) | 5688..7706 | + | 2019 | WP_001795400 | DUF87 domain-containing protein | virb4 |
| HHF57_RS00060 (SAP080A_011) | 7718..8998 | + | 1281 | WP_000702361 | membrane protein | trsF |
| HHF57_RS00065 (SAP080A_012) | 9016..10092 | + | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| HHF57_RS00070 (SAP080A_013) | 10102..10587 | + | 486 | WP_000735569 | TrsH/TraH family protein | - |
| HHF57_RS00075 (SAP080A_014) | 10603..12705 | + | 2103 | WP_001094109 | DNA topoisomerase III | - |
| HHF57_RS00080 (SAP080A_015) | 12721..13185 | + | 465 | WP_000715273 | hypothetical protein | trsJ |
| HHF57_RS00085 (SAP080A_016) | 13182..14822 | + | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HHF57_RS00090 (SAP080A_017) | 14900..15817 | + | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| HHF57_RS00095 (SAP080A_018) | 15834..16226 | + | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| HHF57_RS00100 (SAP080A_019) | 16283..16450 | + | 168 | WP_000869301 | hypothetical protein | - |
| HHF57_RS00105 (SAP080A_020) | 16488..17162 | + | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| HHF57_RS00110 | 17232..17801 | + | 570 | WP_281040023 | sugar phosphate nucleotidyltransferase | - |
| HHF57_RS00115 (SAP080A_022) | 17813..18946 | + | 1134 | WP_000263482 | DegT/DnrJ/EryC1/StrS family aminotransferase | - |
| HHF57_RS00120 (SAP080A_023) | 18931..19692 | + | 762 | WP_002440493 | class I SAM-dependent methyltransferase | - |
Host bacterium
| ID | 801 | GenBank | NC_013343 |
| Plasmid name | SAP080A | Incompatibility group | _ |
| Plasmid size | 43892 bp | Coordinate of oriT [Strand] | 36546..36581 [+] |
| Host baterium | Staphylococcus aureus |
Cargo genes
| Drug resistance gene | aadD, mupA, aac(6')-aph(2'') |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |