Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100338 |
| Name | oriT_SAP069A  |
| Organism | Staphylococcus aureus |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_013339 (24735..24770 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 31..32 |
| Conserved sequence flanking the nic site |
TAAGTGCGCC|CT |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 36 nt
>oriT_SAP069A
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 409 | GenBank | YP_006938388 |
| Name | Nickase_SAP069A |
UniProt ID | O87361 |
| Length | 665 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>YP_006938388.1 oriT nickase (plasmid) [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| PDB | 4HTE | |
| AlphaFold DB | O87361 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4878..16098
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HHF68_RS00010 (SAP069A_002) | 705..1079 | + | 375 | Protein_1 | IS256 family transposase | - |
| HHF68_RS00015 (SAP069A_003) | 1136..1528 | + | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| HHF68_RS00020 (SAP069A_004) | 1529..2968 | + | 1440 | WP_001028144 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| HHF68_RS00025 (SAP069A_005) | 3098..3472 | - | 375 | Protein_4 | IS256 family transposase | - |
| HHF68_RS00030 (SAP069A_006) | 3533..4207 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| HHF68_RS00035 (SAP069A_007) | 4245..4412 | - | 168 | WP_000869301 | hypothetical protein | - |
| HHF68_RS00040 (SAP069A_008) | 4469..4861 | - | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| HHF68_RS00045 (SAP069A_009) | 4878..5795 | - | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| HHF68_RS00050 (SAP069A_010) | 5873..7513 | - | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HHF68_RS00055 (SAP069A_011) | 7510..7974 | - | 465 | WP_000715273 | hypothetical protein | trsJ |
| HHF68_RS00260 | 7990..8337 | - | 348 | WP_309251901 | topoisomerase C-terminal repeat-containing protein | - |
| HHF68_RS00060 (SAP069A_013) | 8413..10091 | - | 1679 | Protein_12 | DNA topoisomerase III | - |
| HHF68_RS00065 (SAP069A_014) | 10107..10592 | - | 486 | WP_000735569 | TrsH/TraH family protein | - |
| HHF68_RS00070 (SAP069A_015) | 10602..11678 | - | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| HHF68_RS00075 (SAP069A_016) | 11696..12976 | - | 1281 | WP_000702361 | membrane protein | trsF |
| HHF68_RS00080 (SAP069A_017) | 12988..15006 | - | 2019 | WP_001795400 | DUF87 domain-containing protein | virb4 |
| HHF68_RS00085 (SAP069A_018) | 15021..15704 | - | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| HHF68_RS00090 (SAP069A_019) | 15691..16098 | - | 408 | WP_000110157 | hypothetical protein | trsC |
| HHF68_RS00095 (SAP069A_020) | 16158..16475 | - | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| HHF68_RS00100 (SAP069A_021) | 16492..17466 | - | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| HHF68_RS00105 (SAP069A_022) | 17638..17826 | + | 189 | WP_001063224 | hypothetical protein | - |
| HHF68_RS00110 (SAP069A_023) | 17935..18609 | - | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| HHF68_RS00115 (SAP069A_024) | 18653..18799 | + | 147 | WP_001817822 | hypothetical protein | - |
| HHF68_RS00120 (SAP069A_025) | 18959..19729 | + | 771 | WP_001795128 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | - |
| HHF68_RS00125 (SAP069A_026) | 20004..20678 | - | 675 | WP_001106022 | IS6-like element IS257 family transposase | - |
Host bacterium
| ID | 799 | GenBank | NC_013339 |
| Plasmid name | SAP069A | Incompatibility group | _ |
| Plasmid size | 42198 bp | Coordinate of oriT [Strand] | 24735..24770 [-] |
| Host baterium | Staphylococcus aureus |
Cargo genes
| Drug resistance gene | aac(6')-aph(2''), aadD, mupA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |