Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100337 |
| Name | oriT_SAP068A  |
| Organism | Staphylococcus aureus |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_013338 (4119..4154 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 31..32 |
| Conserved sequence flanking the nic site |
TAAGTGCGCC|CT |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 36 nt
>oriT_SAP068A
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 408 | GenBank | YP_006938308 |
| Name | Nickase_SAP068A |
UniProt ID | O87361 |
| Length | 665 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>YP_006938308.1 oriT nickase (plasmid) [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| PDB | 4HTE | |
| AlphaFold DB | O87361 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34095..45316
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HHF69_RS00160 (SAP068A_030) | 29193..29867 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| HHF69_RS00165 (SAP068A_031) | 29910..30296 | + | 387 | Protein_32 | IS256 family transposase | - |
| HHF69_RS00170 (SAP068A_032) | 30426..31865 | - | 1440 | WP_001028144 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| HHF69_RS00175 (SAP068A_033) | 31866..32258 | - | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| HHF69_RS00180 (SAP068A_034) | 32315..32689 | - | 375 | Protein_35 | IS256 family transposase | - |
| HHF69_RS00185 (SAP068A_035) | 32750..33424 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| HHF69_RS00190 (SAP068A_036) | 33462..33629 | - | 168 | WP_000869301 | hypothetical protein | - |
| HHF69_RS00195 (SAP068A_037) | 33686..34078 | - | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| HHF69_RS00200 (SAP068A_038) | 34095..35012 | - | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| HHF69_RS00205 (SAP068A_039) | 35090..36730 | - | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HHF69_RS00210 (SAP068A_040) | 36727..37191 | - | 465 | WP_000715273 | hypothetical protein | trsJ |
| HHF69_RS00215 (SAP068A_041) | 37207..39309 | - | 2103 | WP_001094109 | DNA topoisomerase III | - |
| HHF69_RS00220 (SAP068A_042) | 39325..39810 | - | 486 | WP_000735569 | TrsH/TraH family protein | - |
| HHF69_RS00225 (SAP068A_043) | 39820..40896 | - | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| HHF69_RS00230 (SAP068A_044) | 40914..42194 | - | 1281 | WP_000702361 | membrane protein | trsF |
| HHF69_RS00235 (SAP068A_045) | 42206..44224 | - | 2019 | WP_001795400 | DUF87 domain-containing protein | virb4 |
| HHF69_RS00240 (SAP068A_046) | 44239..44922 | - | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| HHF69_RS00245 (SAP068A_047) | 44909..45316 | - | 408 | WP_000110157 | hypothetical protein | trsC |
| HHF69_RS00250 (SAP068A_048) | 45376..45693 | - | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| HHF69_RS00255 (SAP068A_049) | 45710..46684 | - | 975 | WP_012816675 | conjugative transfer protein TrsA | - |
| HHF69_RS00260 (SAP068A_050) | 46856..47044 | + | 189 | WP_001063224 | hypothetical protein | - |
| HHF69_RS00265 (SAP068A_051) | 47153..47827 | - | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| HHF69_RS00270 (SAP068A_052) | 47941..48897 | + | 957 | WP_000282655 | thymidylate synthase | - |
| HHF69_RS00275 (SAP068A_053) | 48939..49424 | + | 486 | WP_000175735 | trimethoprim-resistant dihydrofolate reductase DfrS1 | - |
| HHF69_RS00280 (SAP068A_054) | 49434..49832 | + | 399 | Protein_55 | DegV family protein | - |
Host bacterium
| ID | 798 | GenBank | NC_013338 |
| Plasmid name | SAP068A | Incompatibility group | _ |
| Plasmid size | 50500 bp | Coordinate of oriT [Strand] | 4119..4154 [-] |
| Host baterium | Staphylococcus aureus |
Cargo genes
| Drug resistance gene | blaZ, aac(6')-aph(2'') |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |