Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100334 |
| Name | oriT_pAph01  |
| Organism | Candidatus Accumulibacter phosphatis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_013193 (73854..73960 [-], 107 nt) |
| oriT length | 107 nt |
| IRs (inverted repeats) | 57..75, 78..96 (GTGAAGAAGGACAAGCCGC..GCGGGTGGGCCTACTTCAC) |
| Location of nic site | 104..105 |
| Conserved sequence flanking the nic site |
TATCCTG|C |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 107 nt
>oriT_pAph01
CCGTCCGGCCTCGCAGAGCAGGACGCCCGTTGAGCACGCAGTGCGAATAAGGCGCAGTGAAGAAGGACAAGCCGCTTGCGGGTGGGCCTACTTCACCTATCCTGCCC
CCGTCCGGCCTCGCAGAGCAGGACGCCCGTTGAGCACGCAGTGCGAATAAGGCGCAGTGAAGAAGGACAAGCCGCTTGCGGGTGGGCCTACTTCACCTATCCTGCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 405 | GenBank | WP_012806752 |
| Name | TraI_pAph01 |
UniProt ID | C7RVW0 |
| Length | 741 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 741 a.a. Molecular weight: 81965.40 Da Isoelectric Point: 10.4515
>WP_012806752.1 TraI/MobA(P) family conjugative relaxase [Candidatus Accumulibacter regalis]
MIAKHVPMKSVRKSDFAGLVKYLIDEQQKRERVTGVSVTNCHAERPDAAILEVLNTQAQNRRAESDKTYH
LIVSFRAGEQPDAATLQAIEWRICEGLGYGEHQRVSTVHQDTDNLHLHIAINKIHPTRYTIHDPYRDHKT
LGELCEKLESEYGLQRDNHQAQKRGAENRADDMERHAGIESLLGWIRRECLEQIQGAQSWAELHQALRDN
GLEIRERGNGLVIADAAGTMVKASSVARELSLAKLEARFGRFEPSPERLANQADQPTRQYQPRPFRMRVD
TVELYARYRAEQQGHGAARTVEWTQARDRKSRLIEAAKRSGRLKRAAIKLMGGPGLSRKALYALTSRTLK
DELNQIHQQYRKERQAIHDKYRRQAWADWLRRKATEGDSEALAALRAREAALGLQGNTIAGNGGQQKGQG
VEAQQDGVTKKGTLIYRVGASAIRDDGDKLQVSRGATPDGLEAALRMAMARYGDRITVKGSAEFKESIAH
AAATADLPITFDDAALEQRRQTLLNEITAKENRHDASARTDRGRADRGRIRHPGPNATHPTGAGRRVGSA
AAPRATRKPNVGRVGRQPPPEAQNRLRSLSQLGLVRIASGSEVLLPGHVPDHLEHPGAQPDDGLRRGLAR
PGVAAVAGRAGPAPASSALAAVDKYIAEREEKRLAIFDIPKHRRYNENDSGAAIYAGSRQIDGHSLALLK
RGEEVIVLPINEATARRMQRLALGDPVTVTQGSIKMPGRSR
MIAKHVPMKSVRKSDFAGLVKYLIDEQQKRERVTGVSVTNCHAERPDAAILEVLNTQAQNRRAESDKTYH
LIVSFRAGEQPDAATLQAIEWRICEGLGYGEHQRVSTVHQDTDNLHLHIAINKIHPTRYTIHDPYRDHKT
LGELCEKLESEYGLQRDNHQAQKRGAENRADDMERHAGIESLLGWIRRECLEQIQGAQSWAELHQALRDN
GLEIRERGNGLVIADAAGTMVKASSVARELSLAKLEARFGRFEPSPERLANQADQPTRQYQPRPFRMRVD
TVELYARYRAEQQGHGAARTVEWTQARDRKSRLIEAAKRSGRLKRAAIKLMGGPGLSRKALYALTSRTLK
DELNQIHQQYRKERQAIHDKYRRQAWADWLRRKATEGDSEALAALRAREAALGLQGNTIAGNGGQQKGQG
VEAQQDGVTKKGTLIYRVGASAIRDDGDKLQVSRGATPDGLEAALRMAMARYGDRITVKGSAEFKESIAH
AAATADLPITFDDAALEQRRQTLLNEITAKENRHDASARTDRGRADRGRIRHPGPNATHPTGAGRRVGSA
AAPRATRKPNVGRVGRQPPPEAQNRLRSLSQLGLVRIASGSEVLLPGHVPDHLEHPGAQPDDGLRRGLAR
PGVAAVAGRAGPAPASSALAAVDKYIAEREEKRLAIFDIPKHRRYNENDSGAAIYAGSRQIDGHSLALLK
RGEEVIVLPINEATARRMQRLALGDPVTVTQGSIKMPGRSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | C7RVW0 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 153606..164069
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CAP2UW1_RS22890 (CAP2UW1_4717) | 148701..149780 | - | 1080 | WP_008063278 | signal peptidase II | - |
| CAP2UW1_RS22895 (CAP2UW1_4718) | 149777..152182 | - | 2406 | WP_008063280 | heavy metal translocating P-type ATPase | - |
| CAP2UW1_RS22900 (CAP2UW1_4719) | 152266..152706 | + | 441 | WP_008063281 | Cd(II)/Pb(II)-responsive transcriptional regulator | - |
| CAP2UW1_RS22905 (CAP2UW1_4720) | 152752..152988 | - | 237 | Protein_140 | hypothetical protein | - |
| CAP2UW1_RS22910 (CAP2UW1_4721) | 153007..153591 | - | 585 | WP_012806820 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| CAP2UW1_RS22915 (CAP2UW1_4722) | 153606..155132 | - | 1527 | WP_012806821 | P-type conjugative transfer protein TrbL | virB6 |
| CAP2UW1_RS22920 (CAP2UW1_4723) | 155141..155368 | - | 228 | WP_012806822 | entry exclusion lipoprotein TrbK | - |
| CAP2UW1_RS22925 (CAP2UW1_4724) | 155384..156187 | - | 804 | WP_012806823 | P-type conjugative transfer protein TrbJ | virB5 |
| CAP2UW1_RS22930 (CAP2UW1_4725) | 156206..157645 | - | 1440 | WP_199931765 | TrbI/VirB10 family protein | virB10 |
| CAP2UW1_RS22935 (CAP2UW1_4726) | 157664..158125 | - | 462 | WP_012806825 | conjugal transfer protein TrbH | - |
| CAP2UW1_RS22940 (CAP2UW1_4727) | 158128..159018 | - | 891 | Protein_147 | P-type conjugative transfer protein TrbG | - |
| CAP2UW1_RS22945 (CAP2UW1_4728) | 159032..159805 | - | 774 | WP_012806826 | conjugal transfer protein TrbF | virB8 |
| CAP2UW1_RS22950 (CAP2UW1_4729) | 159802..162360 | - | 2559 | WP_012806827 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| CAP2UW1_RS22955 (CAP2UW1_4730) | 162357..162668 | - | 312 | WP_012806828 | conjugal transfer protein TrbD | virB3 |
| CAP2UW1_RS22960 (CAP2UW1_4731) | 162671..163093 | - | 423 | WP_012806829 | conjugal transfer system pilin TrbC | virB2 |
| CAP2UW1_RS22965 (CAP2UW1_4732) | 163107..164069 | - | 963 | WP_012806830 | P-type conjugative transfer ATPase TrbB | virB11 |
| CAP2UW1_RS22970 (CAP2UW1_4733) | 164393..164764 | - | 372 | WP_012806831 | transcriptional regulator | - |
| CAP2UW1_RS22975 (CAP2UW1_4734) | 165190..166233 | - | 1044 | WP_012806832 | PDDEXK nuclease domain-containing protein | - |
| CAP2UW1_RS22980 (CAP2UW1_4735) | 166246..167226 | - | 981 | WP_012806694 | IS110 family transposase | - |
Host bacterium
| ID | 795 | GenBank | NC_013193 |
| Plasmid name | pAph01 | Incompatibility group | _ |
| Plasmid size | 167595 bp | Coordinate of oriT [Strand] | 73854..73960 [-] |
| Host baterium | Candidatus Accumulibacter phosphatis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | copper resistance |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |