Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100330
Name   oriT_pSRC15 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_013104 (6261..6420 [-], 160 nt)
oriT length   160 nt
IRs (inverted repeats)      IR1: 3..12, 16..25  (CCAGTTTCTC..GAGAAACCGG)
  IR2: 39..44, 48..53  (CCCTAC..GTAGGG)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 160 nt

>oriT_pSRC15
GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGATTGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAGATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   401 GenBank   YP_003104762
Name   MobA_pSRC15 insolico UniProt ID   C7FHN5
Length   709 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 709 a.a.        Molecular weight: 77951.91 Da        Isoelectric Point: 7.2077

>YP_003104762.1 mobilization protein A, MobA (plasmid) [Salmonella enterica subsp. enterica serovar Typhimurium]
MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDAADLYERANGR
LFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQW
FKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGP
NVVEMEGRGIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTG
RRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDA
GFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM
MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGK
TATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDF
IAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGM
DRGGPDFSM

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(410-591)

MobA/MobL family (MobA_MobL)

(37-217)


  Protein structure


Source ID Structure
AlphaFold DB C7FHN5


Host bacterium

ID   791 GenBank   NC_013104
Plasmid name   pSRC15 Incompatibility group   IncQ1
Plasmid size   8688 bp Coordinate of oriT [Strand]   6261..6420 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium

Cargo genes

Drug resistance gene   sul2, aph(3'')-Ib, aph(6)-Id
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -