Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100329 |
| Name | oriT_pKKS825  |
| Organism | Staphylococcus aureus subsp. aureus ST398 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_013034 (9293..9334 [+], 42 nt) |
| oriT length | 42 nt |
| IRs (inverted repeats) | 2..8, 13..19 (ACTTTAT..ATAAAGT) |
| Location of nic site | 27..28 |
| Conserved sequence flanking the nic site |
GTG|TTA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 42 nt
>oriT_pKKS825
CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTACATG
CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTACATG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 400 | GenBank | YP_003084337 |
| Name | Mob_pKKS825 |
UniProt ID | C7C2U8 |
| Length | 409 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 409 a.a. Molecular weight: 48169.25 Da Isoelectric Point: 9.3152
>YP_003084337.1 mobilization protein (plasmid) [Staphylococcus aureus subsp. aureus ST398]
MSMIAARMQKLKAGNLTGIGNHNQRKGQNHSNQEIDPNRSHLNYELVNRTEHYQKDIQTFIDEKKISTRA
VRSDAVLVNEWIITSDRTFFNGLTEQETKEFFEAAKEYFSEKFGEDNIRYATVHVDETTPHMHMGIVPFD
SEGKLSAKRVFNRQTLRDIQEELPTYLQEAKFDIERGQKGSERKNLTVPEFKEMKREQKEVQQETEKMRE
EFRAYVTDRKITVSLDDIKATIEREEIKIPSGEKFLGIDIPKKVERETGNVILSKKDFDKMQDFNTYIKK
SESRFLNLLDTDLYQENKALKKEIKVQKSTNHTNIEDYNQLVREYNTLSGENDRLKSRISDLKKEVRIIY
NTVKSFLKERTSEIQAFKSMFKALTDDISLSSKDNQLDSQFKKEFDKEHTQKRTRGRSR
MSMIAARMQKLKAGNLTGIGNHNQRKGQNHSNQEIDPNRSHLNYELVNRTEHYQKDIQTFIDEKKISTRA
VRSDAVLVNEWIITSDRTFFNGLTEQETKEFFEAAKEYFSEKFGEDNIRYATVHVDETTPHMHMGIVPFD
SEGKLSAKRVFNRQTLRDIQEELPTYLQEAKFDIERGQKGSERKNLTVPEFKEMKREQKEVQQETEKMRE
EFRAYVTDRKITVSLDDIKATIEREEIKIPSGEKFLGIDIPKKVERETGNVILSKKDFDKMQDFNTYIKK
SESRFLNLLDTDLYQENKALKKEIKVQKSTNHTNIEDYNQLVREYNTLSGENDRLKSRISDLKKEVRIIY
NTVKSFLKERTSEIQAFKSMFKALTDDISLSSKDNQLDSQFKKEFDKEHTQKRTRGRSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | C7C2U8 |
Host bacterium
| ID | 790 | GenBank | NC_013034 |
| Plasmid name | pKKS825 | Incompatibility group | _ |
| Plasmid size | 14362 bp | Coordinate of oriT [Strand] | 9293..9334 [+] |
| Host baterium | Staphylococcus aureus subsp. aureus ST398 |
Cargo genes
| Drug resistance gene | resistance to streptogramin A antibiotics, lincosamides, pleuromutilins, kanamycin, neomycin, tetracyclines, andtrimethoprim |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |