Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100320
Name   oriT_pCHE-A in_silico
Organism   Enterobacter cloacae
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_012006 (5063..5150 [-], 88 nt)
oriT length   88 nt
IRs (inverted repeats)      5..10, 16..21  (GTTTCT..AGAAAC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 88 nt

>oriT_pCHE-A
GGCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCGTCAGAATGACGGTCGGGGTTTGCAGCGTCTGTGCTCCGATGATAGCAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   391 GenBank   YP_002563156
Name   MobA_pCHE-A insolico UniProt ID   B9TSG1
Length   701 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 701 a.a.        Molecular weight: 78503.02 Da        Isoelectric Point: 6.6507

>YP_002563156.1 MobA (plasmid) [Enterobacter cloacae]
MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDRDEVLHTQSGHLPKWAERPADYWDVADLYERANGR
LFKEVEFALPVELTLDQQRELVDEFARHLTDGERLPYTLAIHAGDGENPHCHLMISERKNDGIERPADQW
FKRYNAKQPERGGAQKSESLKPKAWLEQTREAWSHHANRALERAGHEARIDHRTLEAQGIERLPGIHLGP
NVVEMEGRGIRTERADTALSIDTANGQIIDLQEYREAIEHERDRQSEEIQRDQRVSGRDRAVGPEHGDTG
GRSPAADRRGTAGQRGAGRELDEPATASHERMAGGSQGHEDSRRGADHGQPDGPERRPWLDVAAMGRSLD
RFRDAYSGAADRIMALAGAARNRATGGHMADFQAQVKSDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM
NREWTPQEVQQNADWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSDDLDAMKQEGREPAAIIETSPRNY
QAWVKVAQDAPAVHRGVIARELAREYDADPASADSRHYGRLAGFTNRKDKHTSRTGYQPWVLCRESSGQI
ATAGPELMQQAGQVLDSIERRQEKARRLEGIEAGPKPSYLRDAVDDYRSEMAGLIKRYGDDLSRCDFIAA
MKLASNGREPDEIAKAMAEASPAIMDRKAGHEADYIQRTLQKVMELPQVQEARAELAKQAEKQQYQGPSC
H

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(415-639)

MobA/MobL family (MobA_MobL)

(23-217)


  Protein structure


Source ID Structure
AlphaFold DB B9TSG1


Host bacterium

ID   782 GenBank   NC_012006
Plasmid name   pCHE-A Incompatibility group   IncQ1
Plasmid size   7560 bp Coordinate of oriT [Strand]   5063..5150 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   beta-lactamase GES-5
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -