Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100319
Name   oriT_pMCCL5 in_silico
Organism   Macrococcoides caseolyticum JCSC5402
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_012000 (4023..4075 [-], 53 nt)
oriT length   53 nt
IRs (inverted repeats)      12..19, 35..42  (GTATATTC..GAATATAC)
Location of nic site      50..51
Conserved sequence flanking the
  nic site    		  CGCTTG|CCA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 53 nt

>oriT_pMCCL5
CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   390 GenBank   WP_000246202
Name   Rlx_pMCCL5 insolico UniProt ID   B9ECE5
Length   320 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 320 a.a.        Molecular weight: 36992.94 Da        Isoelectric Point: 5.4336

>WP_000246202.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Staphylococcaceae]
MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRALYGKENGVQAHTVIQSFKPGEVTAK
ECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHIIINSVNLETGNKYQSNKEQRDFIKKANDQLCEERGL
SVPEKSSEIRYTLAEQNMIDKDKRSWKNDIRMAVEETKDNAVDFEEFNTLLKEKGVEITRVTKNNVTYRH
IEEDKKVRGNKLGDSYDKGVIENGFAIEKFRREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTE
ETKQASNKIYIRDERTTGLERKGIAGNQVEFEKDDGGLSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(8-235)


  Protein structure


Source ID Structure
AlphaFold DB B9ECE5


Host bacterium

ID   781 GenBank   NC_012000
Plasmid name   pMCCL5 Incompatibility group   _
Plasmid size   4398 bp Coordinate of oriT [Strand]   4023..4075 [-]
Host baterium   Macrococcoides caseolyticum JCSC5402

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -