Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100318
Name   oriT_pAtS4e in_silico
Organism   Allorhizobium ampelinum S4
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_011981 (55964..55993 [+], 30 nt)
oriT length   30 nt
IRs (inverted repeats)      15..20, 25..30  (CGTCGC..GCGACG)
Location of nic site      4..5
Conserved sequence flanking the
  nic site    		  AGGG|CGCA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 30 nt

>oriT_pAtS4e
AGGGCGCAATTATACGTCGCTGATGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   389 GenBank   WP_015918238
Name   TraA_pAtS4e insolico UniProt ID   B9K4J7
Length   1100 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123418.63 Da        Isoelectric Point: 9.7244

>WP_015918238.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium/Agrobacterium group]
MAIAHFSASIISRGDGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHQEFVLPADAPNWVRSLIADR
SVSGAVEAFWNKVEAFEKRSDAQLARDLTIALPLELTAEQNIALVRDFVEKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLSEDGFGAKKVAVIGEDGQPVRTKSGKIVYELWAGSTEDFNKLRDGWFERQNHHLALSG
IDLRVDGRSYEKQGIDLEPTIHLGVGAKAIERKAESQGVRPGLERIELNEKRRSENTRRILRRPEIVLDL
ITHEKSVFDEGDISKVLHRYIDDPGMFQQLMARIIQSPDVLRLQRDTIDFATGDKVPARYSTRAMIRLEA
RMATQAIWLSNREGRAVSPTILDETFRRHVRLSDEQRIAIERIAGPARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWGRGRDMLDDRTIFVMDEAGMVASKQMAGFVD
AVVKAGAKIALVGDPEQLQPIEAGAAFRAIVDRIGYAELDSIYRQREDWMRKASLDLARGRVGDALAAYR
SEGRVLGSELKAQAVEHLIADWYRDYDPAKSTLILAHLRRDVGMLNGMARDKLVESGIIGEGHAFRTADG
IRQFDVGDQIVFLKNEGSLGVKNGMIGHVIEAAPNRIVAVVGEGDQRRQVTVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGKRSFAFNGGLSKVLSRRNAKETTLDYERGNLY
REALRYAENRGLNIVQVARTLLRDQLDWTLRQKNRLADLAQRLRAIGERFGLQQSLKTQAMKETAPMIAG
IKTFSGSVADTVEQKLDTDPALKKQWEEVSTRFVYVYADPETAFRAMNFDAVLADKDSARQVLQTLEAEP
AFIGPLKGKTGILASKADREDRRVAELNVPVLKRDLEHYLRMRETAVQRLRADEQVLRQRISIDIPALSP
TAHVVLERVRDAIDRNDLPAAMAYALSNRETKTEIDGFNQALAQRFGERTLLTNAAREPSGKLFDTLSEG
MQPEQKERLKEAWPVMRTAQRLAAHERTVQSLKQSGELRLTQSQTSVLKQ

  Protein domains


Predicted by InterproScan.

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(691-736)

AAA domain (AAA_30)

(382-565)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

MobA/MobL family (MobA_MobL)

(17-243)


  Protein structure


Source ID Structure
AlphaFold DB B9K4J7


Host bacterium

ID   780 GenBank   NC_011981
Plasmid name   pAtS4e Incompatibility group   _
Plasmid size   631775 bp Coordinate of oriT [Strand]   55964..55993 [+]
Host baterium   Allorhizobium ampelinum S4

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   nikB
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9