Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100317 |
| Name | oriT_pAPEC-1  |
| Organism | Escherichia coli chi7122 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_011980 (48127..48416 [+], 290 nt) |
| oriT length | 290 nt |
| IRs (inverted repeats) | 225..232, 235..242 n (GCAAAAAC..GTTTTTGC) |
| Location of nic site | 251..252 |
| Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 290 nt
>oriT_pAPEC-1
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 388 | GenBank | YP_002539341 |
| Name | TraI_pAPEC-1 |
UniProt ID | B9K6C3 |
| Length | 627 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 627 a.a. Molecular weight: 69018.55 Da Isoelectric Point: 5.8819
>YP_002539341.1 TraI (plasmid) [Escherichia coli chi7122]
MAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
PATIISEPDRNVRYARLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVA
SGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQ
EGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNED
RRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGL
ITLEDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSD
GQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVY
TDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFI
APGRKYPQPYVALPAFDRNGKSAGIWLNPLREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD
MAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
PATIISEPDRNVRYARLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVA
SGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQ
EGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNED
RRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGL
ITLEDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSD
GQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVY
TDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFI
APGRKYPQPYVALPAFDRNGKSAGIWLNPLREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B9K6C3 |
Host bacterium
| ID | 779 | GenBank | NC_011980 |
| Plasmid name | pAPEC-1 | Incompatibility group | IncFIC |
| Plasmid size | 103275 bp | Coordinate of oriT [Strand] | 48127..48416 [+] |
| Host baterium | Escherichia coli chi7122 |
Cargo genes
| Drug resistance gene | sitABCD |
| Virulence gene | iroB, iroC, iroD, iroE, iroN, vat, iutA, iucC, iucB, iucA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |