Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100316
Name   oriT_pO26-L in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_011812 (27077..27626 [-], 550 nt)
oriT length   550 nt
IRs (inverted repeats)      243..255, 257..269  (TATTTAATAATTC..GAATTATTAAATA)
 311..318, 321..328  (GCAAAAAC..GTTTTTGC)
Location of nic site      337..338
Conserved sequence flanking the
  nic site    		  GTAGTGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 550 nt

>oriT_pO26-L
TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   387 GenBank   YP_002456225
Name   TraI_pO26-L insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191683.22 Da        Isoelectric Point: 5.6912

>YP_002456225.1 conjugal transfer nickase/helicase TraI (plasmid) [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 26689..50702

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS644_RS00145 (pO26I_p035) 22005..22592 + 588 Protein_29 hypothetical protein -
HS644_RS00150 (pO26I_p036) 22661..23095 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
HS644_RS00155 (pO26I_p037) 23092..23854 + 763 Protein_31 plasmid SOS inhibition protein A -
HS644_RS00480 24033..24182 + 150 Protein_32 plasmid maintenance protein Mok -
HS644_RS00160 (pO26I_p039) 24124..24249 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
HS644_RS00165 (pO26I_p040) 24568..24864 - 297 Protein_34 hypothetical protein -
HS644_RS00170 (pO26I_p041) 25164..25460 + 297 WP_001272251 hypothetical protein -
HS644_RS00175 (pO26I_p042) 25571..26392 + 822 WP_001234445 DUF932 domain-containing protein -
HS644_RS00180 (pO26I_p044) 26689..27291 - 603 WP_000243713 transglycosylase SLT domain-containing protein virB1
HS644_RS00185 (pO26I_p045) 27614..27997 + 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
HS644_RS00190 (pO26I_p046) 28191..28862 + 672 WP_000283561 conjugal transfer transcriptional regulator TraJ -
HS644_RS00195 28999..29226 + 228 WP_000089263 conjugal transfer relaxosome protein TraY -
HS644_RS00200 (pO26I_p047) 29259..29618 + 360 WP_001098992 type IV conjugative transfer system pilin TraA -
HS644_RS00205 (pO26I_p048) 29633..29944 + 312 WP_000012113 type IV conjugative transfer system protein TraL traL
HS644_RS00210 (pO26I_p049) 29966..30532 + 567 WP_000399780 type IV conjugative transfer system protein TraE traE
HS644_RS00215 (pO26I_p050) 30519..31247 + 729 WP_001230772 type-F conjugative transfer system secretin TraK traK
HS644_RS00220 (pO26I_p051) 31247..32698 + 1452 WP_000146675 F-type conjugal transfer pilus assembly protein TraB traB
HS644_RS00225 (pO26I_p052) 32688..33254 + 567 WP_000896599 conjugal transfer pilus-stabilizing protein TraP -
HS644_RS00230 (pO26I_p053) 33241..33561 + 321 WP_001057307 conjugal transfer protein TrbD -
HS644_RS00235 (pO26I_p054) 33558..34073 + 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
HS644_RS00240 (pO26I_p056) 34208..34429 + 222 WP_001278683 conjugal transfer protein TraR -
HS644_RS00245 (pO26I_p057) 34422..34835 + 414 WP_000549589 hypothetical protein -
HS644_RS00250 (pO26I_p058) 34828..35301 + 474 WP_000549568 hypothetical protein -
HS644_RS00255 (pO26I_p059) 35381..35599 + 219 WP_000556745 hypothetical protein -
HS644_RS00260 (pO26I_p060) 35627..35974 + 348 WP_000836682 hypothetical protein -
HS644_RS00265 (pO26I_p061) 36100..38730 + 2631 WP_000069777 type IV secretion system protein TraC virb4
HS644_RS00270 (pO26I_p062) 38727..39113 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
HS644_RS00275 (pO26I_p063) 39110..39742 + 633 WP_001203728 type-F conjugative transfer system protein TraW traW
HS644_RS00280 (pO26I_p064) 39739..40731 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
HS644_RS00285 (pO26I_p065) 40761..41066 + 306 WP_000224416 hypothetical protein -
HS644_RS00290 (pO26I_p066) 41075..41713 + 639 WP_012615298 type-F conjugative transfer system pilin assembly protein TrbC trbC
HS644_RS00295 (pO26I_p067) 41710..43560 + 1851 WP_000821856 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HS644_RS00300 (pO26I_p068) 43587..43844 + 258 WP_000864353 conjugal transfer protein TrbE -
HS644_RS00305 (pO26I_p069) 43837..44580 + 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
HS644_RS00310 (pO26I_p070) 44594..44938 + 345 WP_000556796 conjugal transfer protein TrbA -
HS644_RS00315 (pO26I_p071) 45057..45341 + 285 WP_000624194 type-F conjugative transfer system pilin chaperone TraQ -
HS644_RS00320 (pO26I_p072) 45328..45873 + 546 WP_000059831 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HS644_RS00325 (pO26I_p073) 45803..46150 + 348 WP_001309242 P-type conjugative transfer protein TrbJ -
HS644_RS00330 (pO26I_p074) 46131..46523 + 393 WP_000660699 F-type conjugal transfer protein TrbF -
HS644_RS00335 (pO26I_p075) 46510..47883 + 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
HS644_RS00340 (pO26I_p076) 47880..50702 + 2823 WP_001007039 conjugal transfer mating-pair stabilization protein TraG traG
HS644_RS00345 (pO26I_p077) 50718..51203 + 486 WP_000605870 hypothetical protein -
HS644_RS00350 (pO26I_p078) 51252..51983 + 732 WP_000782451 conjugal transfer complement resistance protein TraT -
HS644_RS00355 (pO26I_p079) 52186..52923 + 738 WP_000199905 hypothetical protein -
HS644_RS00360 52974..53156 + 183 Protein_73 TraD N-terminal domain-containing protein -
HS644_RS00365 (pO26I_p080) 53187..54758 - 1572 WP_000381395 IS66-like element ISCro1 family transposase -
HS644_RS00370 (pO26I_p081) 54778..55125 - 348 WP_000624622 IS66 family insertion sequence element accessory protein TnpB -

Host bacterium

ID   778 GenBank   NC_011812
Plasmid name   pO26-L Incompatibility group   IncFII
Plasmid size   72946 bp Coordinate of oriT [Strand]   27077..27626 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   tet(B)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -